Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, Gloriastrasse 35, CH-8092 Zurich, Switzerland.
Integr Biol (Camb). 2013 Feb;5(2):423-30. doi: 10.1039/c2ib20202f.
Surface-bound self-assembled lipid nanotubes (LNTs) made of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) were used to visualize the contractile activity of spreading cells. The interaction of cells with LNTs resulted in the nucleation of new nanotubes, directed toward the cell center, from existing ones. This process depended on cell generated forces and required acto-myosin mediated contractility. The dynamics of de novo generation of LNTs upon cell spreading was captured using optical microscopy on fluorescently labeled nanotubes and revealed characteristic fingerprints for different cell types such as fibroblasts, endothelial and melanoma cells. Additionally, the method was applied to detect the effect of a specific inhibitor on the generation of cellular forces. The mechanism of the LNT-cell interaction and the potential applications are discussed.
由 1,2-二油酰基-sn-甘油-3-磷酸乙醇胺（DOPE）组成的表面结合的自组装脂质纳米管（LNTs）被用于可视化铺展细胞的收缩活性。细胞与 LNTs 的相互作用导致新的纳米管从现有纳米管中向细胞中心方向成核。这个过程取决于细胞产生的力，并需要肌动球蛋白介导的收缩性。通过在荧光标记的纳米管上使用光学显微镜，捕获细胞铺展时新生成的 LNTs 的动力学，并揭示了不同细胞类型（如成纤维细胞、内皮细胞和黑色素瘤细胞）的特征指纹。此外，该方法还被应用于检测特定抑制剂对细胞力生成的影响。讨论了 LNT-细胞相互作用的机制和潜在应用。
