Sugihara Kaori, Rustom Amin, Spatz Joachim P
Max Planck Institute for Intelligent Systems, Dept. New Materials and Biosystems & University of Heidelberg, Institute for Physical Chemistry, Dept. Biophysical Chemistry, Heisenbergstr. 3, 70569 Stuttgart, Germany.
Soft Matter. 2015 Mar 14;11(10):2029-35. doi: 10.1039/c5sm00043b.
LNTs are unique 3D structures made only of safe and abundant biomaterials by self-assembly. The current bottleneck for developing applications using LNTs is the lack of an easy technique to pattern them on substrates. We report a method to free-draw single lipid nanotube (LNT) patterns in any shape on surfaces with 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) that takes an inverted hexagonal (HII) phase. We used pre-self-assembled LNTs or HII lipid blocks as a lipid reservoir from which new LNTs were pulled by applying a point load with a micromanipulator. The extreme simplicity of our technique originates from the fundamental nature of DOPE lipids that prefer a HII phase, while all the conventional approaches use PC lipids that form a lamellar phase. By adjusting the surface properties with polyelectrolyte multilayers, the created single LNT objects are able to remain adhered to the surface for over a week. Importantly, it could be shown that two vesicles loaded with caged fluorescent molecules were able to fuse well with a LNT, enabling diffusive transport of uncaged fluorescent molecules from one vesicle to the other.
脂质纳米管（LNTs）是通过自组装仅由安全且丰富的生物材料制成的独特三维结构。目前利用LNTs开发应用的瓶颈在于缺乏一种在基底上对其进行图案化的简便技术。我们报道了一种利用处于反相六角（HII）相的1,2 - 二油酰基 - sn - 甘油 - 3 - 磷酸乙醇胺（DOPE）在表面自由绘制任意形状的单个脂质纳米管（LNT）图案的方法。我们使用预自组装的LNTs或HII脂质块作为脂质储备库，通过用微操纵器施加点载荷从中拉出新的LNTs。我们这项技术的极度简便性源于DOPE脂质倾向于HII相的基本性质，而所有传统方法都使用形成层状相的磷脂酰胆碱（PC）脂质。通过用聚电解质多层膜调节表面性质，所创建的单个LNT物体能够在表面附着超过一周。重要的是，可以证明两个装载有笼形荧光分子的囊泡能够与一个LNT很好地融合，从而使未笼化的荧光分子能够从一个囊泡扩散运输到另一个囊泡。
