College of Agriculture, Xinjiang Agricultural University, Xinjiang, China.
Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences/Key Laboratory of Integrated Pest Management on Crops in Northwestern Oasis, Ministry of Agriculture/Scientific Observing and Experimental Station of Korla, Ministry of Agriculture, Xinjiang, China.
J Insect Sci. 2021 Mar 1;21(2). doi: 10.1093/jisesa/ieaa151.
Bemisia tabaci (Gennadius) cryptic complex has invaded Xinjiang, China, since 1998. The distribution of Mediterranean (MED) and Middle East-Asia Minor 1 (MEAM1) B. tabaci substrains has been gradually identified due to the development of molecular technology. In this study, the distribution of MED and MEAM1 in Xinjiang was determined by cleaved amplified polymorphic sequence (CAPs). Results showed that MED dominated in northern Xinjiang (84%), whereas MEAM1 was dominant in southern Xinjiang (72%). Five pairs of simple sequence repeat (SSR) primers were used to analyze the genetic diversity of B. tabaci among 36 geographic populations. The genetic diversity of MED and MEAM1was low and varied little among populations in Xinjiang (0.09 ± 0.14 and 0.09 ± 0.13, respectively). Based on ∆K statistic, 13 populations of MEAM1 could be classified into two subgroups at K = 2, whereas the 23 populations of MED could be classified into four subgroups at K = 4. However, Mantel t-test demonstrated no correlation between geographical and genetic distances among B. tabaci complex (R = 0.42, P = 1.00). Neighbor-joining and principal coordinate analysis showed that geographical isolation and interspecific differences were the main causes of the genetic variation. Gene flow predicted that MEAM1 was most likely introduced from Urumqi to the southern Xinjiang. Meanwhile, a large proportion of MED in Kashi region came from Changji and Yining. To block ongoing dispersal, strict detection and flower quarantine regulations need to be enforced.
烟粉虱（Bemisia tabaci）隐种复合群自 1998 年以来已入侵中国新疆。随着分子技术的发展，逐渐确定了地中海（MED）和中东-中亚 1 型（MEAM1）烟粉虱亚种群的分布。在本研究中，通过切割扩增多态性序列（CAPs）确定了新疆 MED 和 MEAM1 的分布。结果表明，MED 在北疆（84%）占主导地位，而 MEAM1 在南疆（72%）占主导地位。使用 5 对简单重复序列（SSR）引物分析了 36 个地理种群中烟粉虱的遗传多样性。MED 和 MEAM1 的遗传多样性低且新疆种群间差异小（分别为 0.09 ± 0.14 和 0.09 ± 0.13）。基于 ∆K 统计量，可将 MEAM1 的 13 个种群在 K = 2 时分为 2 个亚群，而 23 个 MED 种群在 K = 4 时可分为 4 个亚群。然而，Mantel t 检验表明烟粉虱复合群的地理和遗传距离之间无相关性（R = 0.42，P = 1.00）。邻接聚类和主坐标分析表明，地理隔离和种间差异是遗传变异的主要原因。基因流预测 MEAM1 很可能从乌鲁木齐传入南疆。同时，喀什地区的大量 MED 可能来自昌吉和伊宁。为了阻止正在进行的扩散，需要执行严格的检测和花卉检疫法规。
