Vega Karin, Villena Gretty K, Sarmiento Victor H, Ludeña Yvette, Vera Nadia, Gutiérrez-Correa Marcel
Laboratorio de Micología y Biotecnología, Universidad Nacional Agraria La Molina, Avenida La Molina s/n, Lima 12, Peru.
Biotechnol Res Int. 2012;2012:934325. doi: 10.1155/2012/934325. Epub 2012 Nov 19.
Alkaline cellulase producing fungi were isolated from soils of an undisturbed rain forest of Peru. The soil dilution plate method was used for the enumeration and isolation of fast growing cellulolytic fungi on an enriched selective medium. Eleven out of 50 different morphological colonies were finally selected by using the plate clearing assay with CMC as substrate at different pH values. All 11 strains produced cellulases in liquid culture with activities at alkaline pH values without an apparent decrease of them indicating that they are true alkaline cellulase producers. Aspergillus sp. LM-HP32, Penicillium sp. LM-HP33, and Penicillium sp. LM-HP37 were the best producers of FP cellulase (>3 U mL(-1)) with higher specific productivities (>30 U g(-1) h(-1)). Three strains have been found suitable for developing processes for alkaline cellulase production. Soils from Amazonian rain forests are good sources of industrial fungi with particular characteristics. The results of the present study are of commercial and biological interest. Alkaline cellulases may be used in the polishing and washing of denim processing of the textile industry.
从秘鲁一片未受干扰的雨林土壤中分离出了产碱性纤维素酶的真菌。采用土壤稀释平板法在富集选择性培养基上对生长迅速的纤维素分解真菌进行计数和分离。通过以羧甲基纤维素钠（CMC）为底物、在不同pH值下进行平板透明圈测定，最终从50个不同形态的菌落中筛选出了11个。所有11株菌株在液体培养中均能产生纤维素酶，且在碱性pH值下具有活性，且活性没有明显下降，这表明它们是真正的碱性纤维素酶产生菌。曲霉属LM-HP32、青霉属LM-HP33和青霉属LM-HP37是滤纸纤维素酶（FP纤维素酶）的最佳产生菌（>3 U mL⁻¹），具有较高的比生产率（>30 U g⁻¹ h⁻¹）。已发现三株菌株适合用于开发碱性纤维素酶的生产工艺。亚马逊雨林的土壤是具有特殊特性的工业真菌的良好来源。本研究结果具有商业和生物学意义。碱性纤维素酶可用于纺织工业牛仔布加工的抛光和洗涤。
