[反相高效液相色谱法测定不同产地虎刺中齐墩果酸和熊果酸的含量]
[Determination of oleanolic acid and ursolic acid in Damnacanthus indicus from different places by RP-hPLC].
作者信息
Cai Ye-fen, Huang Qing-song
机构信息
Department of Pharmacy, Guangzhou Medical University Cancer Institute Hospital, Guangzhou 510095, China.
出版信息
Zhong Yao Cai. 2012 May;35(5):694-6.
OBJECTIVE
To establish A RP-HPLC method for determination of oleanolic acid and ursolic acid in Damnacanthus indicus from different places.
METHODS
After ultrasonic extraction with 95% ethanol,the separation was achieved on a Kromasil C18 column at 25 degrees C using methanol-2.0% phosphoric acid solution (90:10, v/v) as the mobile phase at a flow rate of 0.8 mL/min. The detection wavelength was 210 nm.
RESULTS
Oleanolic acid showed good linear at the range of 0.124-1.24 microg (r = 0.9997). The recovery was 97.6% and RSD was 2.2%. Ursolic acid showed good linear at the range of 0.192-1.92 microg (r = 0.9999). The recovery was 102.4% and RSD was 1.9%.
CONCLUSION
The proposed method is simple and accurate. It can provide reliable evidence for quality control, development and utilization of Damnacanthus indicus.
目的
建立测定不同产地虎刺中齐墩果酸和熊果酸含量的反相高效液相色谱法。
方法
采用95%乙醇超声提取后，以甲醇-2.0%磷酸溶液（90:10，v/v）为流动相，流速为0.8 mL/min，在Kromasil C18柱上于25℃进行分离，检测波长为210 nm。
结果
齐墩果酸在0.124 - 1.24 μg范围内线性关系良好（r = 0.9997），回收率为97.6%，相对标准偏差为2.2%。熊果酸在0.192 - 1.92 μg范围内线性关系良好（r = 0.9999），回收率为102.4%，相对标准偏差为1.9%。
结论
该方法简便、准确，可为虎刺的质量控制、开发利用提供可靠依据。
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