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[不同炮制方法对菊花黄酮含量的影响]

[The influence of different processing on the content of flavones of Dendranthema morifolium].

作者信息

Jin Chuan-shan, Wu De-lin, Yu Nian-jun, Chen Hui-fang, Wang Tong-sheng, Zhang Wei

机构信息

College of Pharmaceutical, Anhui university of traditional Chinese medicine, Hefei 230031, China.

出版信息

Zhong Yao Cai. 2012 May;35(5):702-4.

Abstract

OBJECTIVE

To investigate the effect of different processing methods on the content of luteolin-7-O-beta-D-glucoside, acacetin-7-O-beta-D-glucoside, luteolin, and acacetin of Dendranthema morifolium.

METHODS

The optimal conditions were achieved on phenomenex, synergi Fusion-RP (5 microm, 4.6 mm x 250 mm)analytical column with a gradient mobile phase consisting of methanol and 0.1% phosphoric acid and detection wavelength set at 350 nm.

RESULTS

The significant effect of different processing methods on the content of luteolin-7-O-beta-D-glucoside, acacetin-7-O-beta-D-glucoside, luteolin and acacetin was proved.

CONCLUSION

Different processing methods have significant effect on flavonoids of Dendranthema morifolium, it should be paid attention to drying temperature while sulphur fumigation.

摘要

目的

考察不同炮制方法对菊花中木犀草素-7-O-β-D-葡萄糖苷、刺槐素-7-O-β-D-葡萄糖苷、木犀草素及刺槐素含量的影响。

方法

采用phenomenex,synergi Fusion-RP(5μm,4.6mm×250mm)分析柱,以甲醇和0.1%磷酸为流动相进行梯度洗脱,检测波长为350nm,在此条件下获得最佳结果。

结果

证明了不同炮制方法对木犀草素-7-O-β-D-葡萄糖苷、刺槐素-7-O-β-D-葡萄糖苷、木犀草素及刺槐素含量有显著影响。

结论

不同炮制方法对菊花黄酮类成分有显著影响,硫磺熏蒸时应注意干燥温度。

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