Jin Chuan-shan, Wu De-lin, Yu Nian-jun, Chen Hui-fang, Wang Tong-sheng, Zhang Wei
College of Pharmaceutical, Anhui university of traditional Chinese medicine, Hefei 230031, China.
Zhong Yao Cai. 2012 May;35(5):702-4.
To investigate the effect of different processing methods on the content of luteolin-7-O-beta-D-glucoside, acacetin-7-O-beta-D-glucoside, luteolin, and acacetin of Dendranthema morifolium.
The optimal conditions were achieved on phenomenex, synergi Fusion-RP (5 microm, 4.6 mm x 250 mm)analytical column with a gradient mobile phase consisting of methanol and 0.1% phosphoric acid and detection wavelength set at 350 nm.
The significant effect of different processing methods on the content of luteolin-7-O-beta-D-glucoside, acacetin-7-O-beta-D-glucoside, luteolin and acacetin was proved.
Different processing methods have significant effect on flavonoids of Dendranthema morifolium, it should be paid attention to drying temperature while sulphur fumigation.
考察不同炮制方法对菊花中木犀草素-7-O-β-D-葡萄糖苷、刺槐素-7-O-β-D-葡萄糖苷、木犀草素及刺槐素含量的影响。
采用phenomenex,synergi Fusion-RP(5μm,4.6mm×250mm)分析柱,以甲醇和0.1%磷酸为流动相进行梯度洗脱,检测波长为350nm,在此条件下获得最佳结果。
证明了不同炮制方法对木犀草素-7-O-β-D-葡萄糖苷、刺槐素-7-O-β-D-葡萄糖苷、木犀草素及刺槐素含量有显著影响。
不同炮制方法对菊花黄酮类成分有显著影响,硫磺熏蒸时应注意干燥温度。