Zhu Wen-xiu, Huang Zhen-xing, Ren Hong-yan, Ruan Wen-quan
School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China.
Huan Jing Ke Xue. 2012 Aug;33(8):2715-22.
A lab-scale internal circulation reactor (IC) fed by artificial brewery wastewater was operated with increasing volumetric loading rate under 35 degrees C continuously. The reactor performance and the relationship between microbial community structure and bioactivity in the anaerobic sludge were investigated during the operation. The COD removal efficiency was above 85%, furthermore, the maximum volumetric loading rate (VLR) and the maximum specific methanogenic activity (SMA) of the reactor could be up to 20 kg x (m3 x d)(-1) and 210 mL x (g x d)(-1) respectively. The results from the dehydrogenase and the bacteria DGGE experiments demonstrated that the dehydrogenase variation tendency was positively correlated to total light intensity of the whole bacteria DGGE bands for each sample. The total light intensity of the whole bacteria DGGE bands can use as a referential index for biomass liveweight in anaerobic system. Moreover, the coenzyme F420 content related to the relative abundance of Methanosaeta based on coenzyme F420 and archaebacteria DGGE analysis. As the volumetric loading rate increased, Methanomsaeta became significantly dominant, which was accompanied by the coenzyme F420 content increasing. The content could be up to 0.16 micromol x g(-1), meanwhile, the superiority of Methanosaeta became significantly obvious; UPGAMA analysis and Shannon index also confirmed the dynamic changes of microbial community structure during the operation.
一个由人工酿造废水进料的实验室规模的内循环反应器（IC）在35℃下连续运行，容积负荷率不断增加。在运行过程中，研究了反应器性能以及厌氧污泥中微生物群落结构与生物活性之间的关系。化学需氧量（COD）去除效率高于85%，此外，该反应器的最大容积负荷率（VLR）和最大比产甲烷活性（SMA）分别可达20 kg·(m³·d)⁻¹和210 mL·(g·d)⁻¹。脱氢酶和细菌变性梯度凝胶电泳（DGGE）实验结果表明，每个样品中脱氢酶的变化趋势与整个细菌DGGE条带的总光强度呈正相关。整个细菌DGGE条带的总光强度可作为厌氧系统中生物量活重的参考指标。此外，基于辅酶F420和古细菌DGGE分析，辅酶F420含量与甲烷八叠球菌的相对丰度有关。随着容积负荷率的增加，甲烷八叠球菌变得显著占优势，同时辅酶F420含量增加。含量可达0.16 μmol·g⁻¹，与此同时，甲烷八叠球菌的优势变得非常明显；非加权组平均法（UPGAMA）分析和香农指数也证实了运行过程中微生物群落结构的动态变化。
