College of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University , 103 Wenhua Road, Shenyang 110016, People's Republic of China.
J Agric Food Chem. 2012 Dec 26;60(51):12574-83. doi: 10.1021/jf304080v. Epub 2012 Dec 14.
A multicomponent quantification fingerprint based on HPLC coupled with diode array detection and electrospray ionization tandem mass spectrometry (HPLC-DAD-ESI/MS) technique has been established for the analysis of phenolic compounds in 12 samples originated from 5 different cultivars of Chrysanthemum morifolium flowers in China. Four caffeoylquinic acids and 15 flavonoids in the capitulum were identified by comparing the retention times and ultraviolet spectra as well as the mass spectrum and/or matching the empirical molecular formula with that of reference compounds, and the contents of these compounds have been determined simultaneously. The samples from three medicinal cultivars significantly differed in the quality and quantity of flavonoid aglycones and glycosides compared with those from two edible cultivars, which allows the possibility of showing the chemical distinctness of these cultivars and may be useful in their standardization. Moreover, the antiallergic effects of these cultivars were comparatively assayed for the first time. A representative medicinal cultivar, 'huaiju', showed potential activity on the inhibition of antigen-induced degranulation from RBL-2H3 cells and compound 48/80-induced scratching in mice, whereas the in vitro and in vivo antiallergic activities of two edible cultivars were weak. The results suggested that the quality and quantity of some active flavonoid aglycones should be responsible for the pharmacological profiles of these cultivars.
建立了一种基于 HPLC 与二极管阵列检测和电喷雾串联质谱联用（HPLC-DAD-ESI/MS）技术的多组分定量指纹图谱，用于分析来自中国 5 个不同品种的 12 个菊花头状花序中的酚类化合物。通过比较保留时间、紫外光谱以及质谱和/或与对照化合物的经验分子式相匹配，鉴定了 4 种咖啡酰奎宁酸和 15 种黄酮类化合物，同时测定了这些化合物的含量。与 2 种食用品种相比，3 种药用品种的类黄酮苷元和糖苷的质量和含量明显不同，这表明这些品种在化学成分上存在差异，可能有助于其标准化。此外，首次比较了这些品种的抗过敏作用。代表性药用品种“怀菊”在抑制 RBL-2H3 细胞抗原诱导脱颗粒和化合物 48/80 诱导小鼠搔抓方面表现出潜在的活性，而 2 种食用品种的体外和体内抗过敏活性较弱。结果表明，一些活性类黄酮苷元的质量和数量可能是这些品种药理作用的原因。
