Akalın-Evren Buket, Kulak-Özkan Yasemin, Ozcan Mutlu, Kadir Tanju
Department of Prosthodontics, Faculty of Dentistry, University of Marmara, Istanbul, Turkey.
Gerodontology. 2014 Sep;31(3):194-201. doi: 10.1111/ger.12024. Epub 2012 Dec 5.
Fiber-reinforced composites (FRC) are used to reinforce the prosthetic and restorative appliances. This may result in the exposure of the FRCs which may affect the adherence of microorganisms. This study evaluated the adhesion of Candida albicans to a denture base resin (N = 48), reinforced with E-glass FRCs with different architectures [unidirectional (n = 16) and woven (n = 16)], when exposed to either saliva or distilled water.
Specimens without FRC reinforcement served as control (n = 16). After fiber surfaces were exposed, half of the specimens (n = 8/per group) were treated with saliva, the other half was stored in distilled water prior to C. albicans adhesion. The adhered microorganisms were counted under an optical microscope and the data were analyzed.
When exposed to distilled water or saliva, specimens with unidirectional (5403.4 cells/cm(2) and 5013.4 cells/cm(2) , respectively) (p = 0.202), woven fibers (4053.5 cells/cm(2) and 3726.6 cells/cm(2) , respectively) (p = 0.283) and specimens without fibers (2250 cells/cm(2) and 2006.8 cells/cm(2) , respectively) (p = 0.423) showed C. albicans adhesion, being not significant. In general, all the specimens exposed to saliva showed 3582.2 cells/cm(2) C. albicans adhesion, while specimens exposed to distilled water showed 3902 cells/cm(2) C. albicans adhesion, yet being not significant (p = 0.436). Regarding fiber type, C. albicans adhesion was significantly affected by the exposed FRC architecture with more favorable results for woven fibers (p < 0.001).
Polymethylmethacrylate without FRCs collected less C. albicans. The presence of saliva seems to reduce the adhesion.
纤维增强复合材料（FRC）用于增强修复体和矫治器。这可能导致FRC暴露，进而可能影响微生物的附着。本研究评估了白色念珠菌在暴露于唾液或蒸馏水时，对用不同结构的E玻璃纤维增强的义齿基托树脂（N = 48）的黏附情况，其中不同结构包括单向（n = 16）和编织（n = 16）。
未用FRC增强的标本作为对照（n = 16）。纤维表面暴露后，一半标本（每组n = 8）用唾液处理，另一半在白色念珠菌黏附前储存在蒸馏水中。在光学显微镜下对黏附的微生物进行计数并分析数据。
暴露于蒸馏水或唾液时，单向纤维标本（分别为5403.4个细胞/cm²和5013.4个细胞/cm²）（p = 0.202）、编织纤维标本（分别为4053.5个细胞/cm²和3726.6个细胞/cm²）（p = 0.283）以及无纤维标本（分别为2250个细胞/cm²和2006.8个细胞/cm²）（p = 0.423）均显示有白色念珠菌黏附，但差异无统计学意义。总体而言，所有暴露于唾液的标本显示白色念珠菌黏附量为3582.2个细胞/cm²，而暴露于蒸馏水的标本显示白色念珠菌黏附量为3902个细胞/cm²，差异也无统计学意义（p = 0.436）。关于纤维类型，白色念珠菌黏附受暴露的FRC结构显著影响，编织纤维的结果更有利（p < 0.001）。
不含FRC的聚甲基丙烯酸甲酯收集到的白色念珠菌较少。唾液的存在似乎会降低黏附。
