Zamperini Camila A, Schiavinato Patricia C S, Pavarina Ana C, Giampaolo Eunice T, Vergani Carlos E, Machado Ana L
Department of Dental Materials and Prosthodontics, Araraquara Dental School, São Paulo State University - UNESP, São Paulo, Brazil.
J Investig Clin Dent. 2013 Nov;4(4):225-8. doi: 10.1111/jicd.12006. Epub 2012 Dec 17.
In studies on Candida albicans adhesion to surfaces, diverse protocols have been used for collection and preparation of saliva samples. Thus, this study investigated whether variations in the centrifugation parameters and number of donors of saliva would influence the adhesion of C. albicans to a denture base resin.
Resin acrylic samples (n = 72) were made and then divided into four groups: (a) control - specimens were left without preconditioning in saliva; (b) three experimental groups, in which the specimens were preconditioned with saliva collected from 15 volunteers and centrifuged at 12 000 g for 5 min (G1 ); from 15 volunteers and centrifuged at 18 000 g for 30 min (G2 ); and from one volunteer and centrifuged at 12 000 g for 5 min (G3 ). Candida adhesion was evaluated by both the 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide (XTT) reduction method and crystal violet staining. Data were analyzed by one-way analyses of variance (P = 0.05).
For XTT reduction assay, groups G2 , G3 , and control were not significantly different, whereas group G1 showed significantly higher absorbance value than control. For crystal violet staining there were no significant differences among all groups.
Variations in the centrifugation parameters and number of donors of saliva may influence C. albicans adhesion to denture base resins.
在白色念珠菌黏附于表面的研究中，已采用多种方案来采集和制备唾液样本。因此，本研究调查了离心参数和唾液供体数量的变化是否会影响白色念珠菌对义齿基托树脂的黏附。
制作丙烯酸树脂样本（n = 72），然后将其分为四组：（a）对照组——样本未用唾液进行预处理；（b）三个实验组，其中样本分别用从15名志愿者采集的唾液进行预处理，并在12 000 g下离心5分钟（G1组）；从15名志愿者采集的唾液并在18 000 g下离心30分钟（G2组）；以及从一名志愿者采集的唾液并在12 000 g下离心5分钟（G3组）。通过2,3-双（2-甲氧基-4-硝基-5-磺基苯基）-5-[(苯胺基)羰基]-2H-氢氧化四唑（XTT）还原法和结晶紫染色评估念珠菌的黏附情况。数据采用单因素方差分析进行分析（P = 0.05）。
对于XTT还原试验，G2组、G3组和对照组之间无显著差异，而G1组的吸光度值显著高于对照组。对于结晶紫染色，所有组之间均无显著差异。
离心参数和唾液供体数量的变化可能会影响白色念珠菌对义齿基托树脂的黏附。
