Shirley Nicholas, Allgeier Lindsay, Lanier Tommy, Coyle Heather Miller
Forensic Science Department, Henry C. Lee College of Criminal Justice & Forensic Sciences, University of New Haven, 300 Boston Post Road, West Haven, CT 06516, USA.
J Forensic Sci. 2013 Jan;58 Suppl 1:S176-82. doi: 10.1111/1556-4029.12005. Epub 2012 Dec 6.
We have analyzed the distribution of genotypes at a single hexanucleotide short tandem repeat (STR) locus in a Cannabis sativa seed database along with seed-packaging information. This STR locus is defined by the polymerase chain reaction amplification primers CS1F and CS1R and is referred to as NMI01 (for National Marijuana Initiative) in our study. The population database consists of seed seizures of two categories: seed samples from labeled and unlabeled packages regarding seed bank source. Of a population database of 93 processed seeds including 12 labeled Cannabis varieties, the observed genotypes generated from single seeds exhibited between one and three peaks (potentially six alleles if in homozygous state). The total number of observed genotypes was 54 making this marker highly specific and highly individualizing even among seeds of common lineage. Cluster analysis associated many but not all of the handwritten labeled seed varieties tested to date as well as the National Park seizure to our known reference database containing Mr. Nice Seedbank and Sensi Seeds commercially packaged reference samples.
我们结合种子包装信息，分析了大麻种子数据库中单个六核苷酸短串联重复序列（STR）位点的基因型分布。该STR位点由聚合酶链反应扩增引物CS1F和CS1R定义，在我们的研究中被称为NMI01（国家大麻计划）。种群数据库由两类种子查获样本组成：来自有标签和无标签包装的种子样本，涉及种子库来源。在一个包含93个处理种子的种群数据库中，其中有12个有标签的大麻品种，从单粒种子产生的观察到的基因型显示出1到3个峰（如果处于纯合状态可能有6个等位基因）。观察到的基因型总数为54种，这使得该标记即使在常见谱系的种子中也具有高度特异性和高度个体化。聚类分析将许多但不是所有迄今为止测试的手写标签种子品种以及国家公园查获的种子与我们已知的参考数据库相关联，该参考数据库包含商业包装的“好人先生种子库”和“森西种子”参考样本。
