米诺环素可减少脑积水大鼠模型中的反应性神经胶质增生。

Minocycline reduces reactive gliosis in the rat model of hydrocephalus.

机构信息

Department of Neurosurgery, First Affiliate Hospital of Xiamen University, Xiamen, Fujian Province 361003, China.

出版信息

BMC Neurosci. 2012 Dec 5;13:148. doi: 10.1186/1471-2202-13-148.

BACKGROUND

Reactive gliosis had been implicated in injury and recovery patterns associated with hydrocephalus. Our aim is to determine the efficacy of minocycline, an antibiotic known for its anti-inflammatory properties, to reduce reactive gliosis and inhibit the development of hydrocephalus.

RESULTS

The ventricular dilatation were evaluated by MRI at 1-week post drugs treated, while GFAP and Iba-1were detected by RT-PCR, Immunohistochemistry and Western blot. The expression of GFAP and Iba-1 was significantly higher in hydrocephalic group compared with saline control group (p < 0.05). Minocycline treatment of hydrocephalic animals reduced the expression of GFAP and Iba-1 significantly (p < 0.05). Likewise, the severity of ventricular dilatation is lower in minocycline treated hydrocephalic animals compared with the no minocycline group (p < 0.05).

CONCLUSION

Minocycline treatment is effective in reducing the gliosis and delaying the development of hydrocephalus with prospective to be the auxiliary therapeutic method of hydrocephalus.

背景

反应性神经胶质增生与脑积水相关的损伤和恢复模式有关。我们的目的是确定米诺环素（一种以其抗炎特性而闻名的抗生素）的功效，以减少反应性神经胶质增生并抑制脑积水的发展。

结果

在药物治疗后 1 周通过 MRI 评估脑室扩张，同时通过 RT-PCR、免疫组织化学和 Western blot 检测 GFAP 和 Iba-1。与盐水对照组相比，脑积水组的 GFAP 和 Iba-1 表达显著升高（p<0.05）。米诺环素治疗脑积水动物可显著降低 GFAP 和 Iba-1 的表达（p<0.05）。同样，米诺环素治疗的脑积水动物的脑室扩张严重程度低于无米诺环素组（p<0.05）。