Department of Surgery, The Third Affiliated (Tumor) Hospital, Harbin Medical University, Harbin 150040, China.
Carbohydr Polym. 2013 Jan 30;92(1):577-82. doi: 10.1016/j.carbpol.2012.09.006. Epub 2012 Sep 13.
A neutral water-soluble polysaccharide (W-NTRP), with a molecular weight of 2.5 × 10(4)Da, was isolated from the fruit bodies of Trametes robiniophila (Huaier). Gas chromatography (GC) results indicated that W-NTRP was determined to be galactose (Gal), arabinose (Ara) and glucose (Glc), with a relative molar ratio of 4.2:2.5:0.7. Its antitumor and immunomodulatory activity were evaluated in vitro. W-NTRP showed remarkable inhibitory effect on three human cholangiocarcinoma cell lines (QBC939, Sk-ChA-1 and MZ-ChA-1), with respective IC(50) values of 47.8, 75.9, and 43.7 μg/mL, but had no cytotoxicity to L-929 normal cells. Furthermore, W-NTRP had proliferation promoting effect on mouse splenocytes with or without concanavalin A (ConA) or lipopolysaccharide (LPS) in a bell-shaped dose-response manner. In addition, W-NTRP could prominently stimulate macrophages to produce nitric oxide (NO) through the up-regulation of inducible NO synthase (iNOS) activity. These results suggest that W-NTRP could be explored as a potential antitumor agent for cholangiocarcinoma.
一种中性水溶性多糖（W-NTRP），分子量为 2.5×10(4)Da，从中华被毛孢（Huaier）的子实体中分离得到。气相色谱（GC）结果表明，W-NTRP 被确定为半乳糖（Gal）、阿拉伯糖（Ara）和葡萄糖（Glc），相对摩尔比为 4.2:2.5:0.7。体外评价了其抗肿瘤和免疫调节活性。W-NTRP 对三种人胆管癌细胞系（QBC939、Sk-ChA-1 和 MZ-ChA-1）表现出显著的抑制作用，其 IC(50)值分别为 47.8、75.9 和 43.7 μg/mL，但对 L-929 正常细胞无细胞毒性。此外，W-NTRP 以钟形剂量反应方式对有或没有刀豆球蛋白 A（ConA）或脂多糖（LPS）的小鼠脾细胞具有增殖促进作用。此外，W-NTRP 可通过诱导型一氧化氮合酶（iNOS）活性的上调，显著刺激巨噬细胞产生一氧化氮（NO）。这些结果表明，W-NTRP 可作为胆管癌的潜在抗肿瘤药物进行探索。
