蛹虫草菌丝体多糖硫酸酯化修饰及其生物活性。
Sulfated modification of the polysaccharide from Cordyceps_gunnii mycelia and its biological activities.
机构信息
Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
出版信息
Carbohydr Polym. 2013 Jan 30;92(1):872-6. doi: 10.1016/j.carbpol.2012.10.007. Epub 2012 Oct 12.
A chemically new sulfated polysaccharide (SPS50) was prepared from the water soluble polysaccharide (PS50), isolated from Cordyceps_gunnii mycelia, by concentrated sulfuric acid method. The yield of crude SPS50 was 62.34% and its specific rotation was α(20)=-36.75°. The structural characteristics of this chemically sulfated polysaccharide were determined based on the infrared analysis (IR), high performance liquid chromatography (HPLC) and sodium dodecyl sulfate polyacrylamide gel electropheresis (SDS-PAGE). Its biological properties including anti-oxidant and anti-tumor activities were also investigated. The results showed that the anti-oxidant capacity of SPS50 was not as good as SP50 and the anti-tumor activity of SPS50 was much better than PS50. SPS50 showed evident growth inhibition on K562 cells. The tumor inhibition ratio of SPS50 against K562 cells was 69.92%.
一种新型硫酸化多糖（SPS50）是由蛹虫草水溶性多糖（PS50）经浓硫酸法制备得到的。粗 SPS50 的产率为 62.34%，比旋光度[α]（D）（20）=-36.75°。通过红外分析（IR）、高效液相色谱（HPLC）和十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）确定了该化学硫酸化多糖的结构特征。还研究了其包括抗氧化和抗肿瘤活性在内的生物特性。结果表明，SPS50 的抗氧化能力不如 SP50，而 SPS50 的抗肿瘤活性明显优于 PS50。SPS50 对 K562 细胞表现出明显的生长抑制作用。SPS50 对 K562 细胞的肿瘤抑制率为 69.92%。
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