黄芪多糖对高迁移率族蛋白 B1 诱导的内皮细胞通透性的研究。

Study of Astragalus mongholicus polysaccharides on endothelial cells permeability induced by HMGB1.

机构信息

Emergency Department, Chongming Branch, Xin Hua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 202150, China.

出版信息

Carbohydr Polym. 2013 Jan 30;92(1):934-41. doi: 10.1016/j.carbpol.2012.08.001. Epub 2012 Sep 12.

DOI:10.1016/j.carbpol.2012.08.001

PMID:23218386

Abstract

Astragalus mongholicus polysaccharide (APS) shows various biological activities. Here, we explored the effect of APS on high mobility group protein 1 (HMGB1) -induced endothelial cell permeability. The results indicated APS pretreatment effectively inhibited HMGB1-induced increased permeability in endothelial cells (ECs). Signal transduction studies showed APS inhibited not only the activation of small guanylate Rho and its downstream effector Rho kinase (ROCK), but also the subsequent phosphorylation of myosin light chain (MLC) in ECs. In conclusion, our investigations suggested that APS inhibited HMGB1-induced increased permeability in ECs, regulated by Rho/ROCK signal pathways.

摘要

蒙古黄芪多糖 (APS) 具有多种生物学活性。在这里，我们研究了 APS 对高迁移率族蛋白 1 (HMGB1) 诱导的内皮细胞通透性的影响。结果表明，APS 预处理可有效抑制 HMGB1 诱导的内皮细胞通透性增加。信号转导研究表明，APS 不仅抑制了小 G 蛋白 Rho 的激活及其下游效应物 Rho 激酶 (ROCK)，还抑制了 Rho/ROCK 信号通路下游 ECs 中肌球蛋白轻链 (MLC)的磷酸化。总之，我们的研究表明，APS 通过 Rho/ROCK 信号通路抑制 HMGB1 诱导的内皮细胞通透性增加。