Department of Gynaecology, Federal University of São Paulo, São Paulo, Brazil.
Eur J Obstet Gynecol Reprod Biol. 2013 Mar;167(1):47-52. doi: 10.1016/j.ejogrb.2012.10.021. Epub 2012 Dec 4.
To investigate expression of BcL-2, FAS, FAS ligand (FASL) and cleaved caspase-3 in the endometrial tissue of women with idiopathic infertility (with two consecutive failed cycles of in vitro fertilization) and women with idiopathic recurrent pregnancy loss. The control group consisted of fertile women.
Endometrial tissue samples from fertile women (n=25), women with idiopathic infertility (n=25) and women with idiopathic recurrent pregnancy loss (n=25) were collected on the seventh or eighth postovulatory day of their menstrual cycles for evaluation. Expression of BcL-2, FAS, FASL and cleaved caspase-3 was assessed using immunohistochemical methods.
Expression of BcL-2 and FAS was significantly higher and lower, respectively, in the women with idiopathic infertility than in the other groups (p<0.01). Expression of cleaved caspase-3 was significantly lower in the women with idiopathic infertility than in the other groups (p<0.01). Expression of FASL was similar in all three groups.
Disturbances in endometrial apoptosis may be a contributing factor in patients with idiopathic infertility and recurrent pregnancy loss.
研究不明原因不孕（连续两次体外受精失败周期）和不明原因复发性流产患者子宫内膜组织中 BcL-2、FAS、FAS 配体（FASL）和 cleaved caspase-3 的表达。对照组为生育力正常的妇女。
收集生育力正常妇女（n=25）、不明原因不孕妇女（n=25）和不明原因复发性流产妇女（n=25）在月经周期的第 7 或第 8 天排卵后的子宫内膜组织样本进行评估。采用免疫组织化学方法检测 BcL-2、FAS、FASL 和 cleaved caspase-3 的表达。
与其他两组相比，不明原因不孕妇女的 BcL-2 表达显著升高，FAS 表达显著降低（p<0.01）。不明原因不孕妇女的 cleaved caspase-3 表达显著低于其他两组（p<0.01）。三组间 FASL 的表达相似。
子宫内膜细胞凋亡紊乱可能是不明原因不孕和复发性流产患者的一个致病因素。
