Zellbiologie, Universität Bayreuth, 95440 Bayreuth, Germany.
Eur J Cell Biol. 2013 Feb;92(2):80-6. doi: 10.1016/j.ejcb.2012.10.004. Epub 2012 Dec 3.
Mitochondria are dynamic organelles that were found to fuse and divide in many different cell types. Mitochondrial fusion plays important roles in maintenance of respiratory capacity, dissipation of metabolic energy, and inheritance of mitochondrial DNA. While the molecular machinery of mitochondrial fusion has been characterized in great detail in yeast and mammals, only little is known about mitochondrial fusion in higher plants and algae. We asked whether mitochondrial fusion can be observed in the unicellular green alga Chlamydomonas reinhardtii. Mitochondria were stained with fluorescent dyes in gametes, and mixing of fluorescent markers was detected by fluorescence microscopy in zygotes indicating fusion. Mitochondrial fusion was observed in wild type zygotes, and also in respiratory mutants, albeit with less efficiency. We conclude that mitochondria readily fuse in green algae.
线粒体是动态细胞器，在许多不同类型的细胞中被发现融合和分裂。线粒体融合在维持呼吸能力、代谢能量耗散和线粒体 DNA 的遗传方面起着重要作用。虽然酵母和哺乳动物中线粒体融合的分子机制已经被详细描述，但关于高等植物和藻类中的线粒体融合知之甚少。我们想知道在单细胞绿藻衣藻中是否可以观察到线粒体融合。用荧光染料对配子中的线粒体进行染色，并通过荧光显微镜在合子中检测荧光标记物的混合来指示融合。在野生型合子中观察到线粒体融合，在呼吸突变体中也观察到了线粒体融合，尽管效率较低。我们的结论是，线粒体在绿藻中很容易融合。
