Department of Pathology and Parasitology, University of Veterinary and Pharmaceutical Sciences, Palackého 1-3, 612 42 Brno, Czech Republic.
Vet Parasitol. 2013 Mar 31;193(1-3):95-9. doi: 10.1016/j.vetpar.2012.11.016. Epub 2012 Nov 14.
Undercooked meat containing tissue cysts is one of the most common sources of Toxoplasma gondii infection in humans. Goats are very susceptible to clinical toxoplasmosis, and especially kids are common food animals, thereby representing a risk for human infection. A sequence-specific magnetic capture method was used for isolation of T. gondii DNA from tissue samples from experimentally infected goat-kids and real-time PCR for the 529 bp repeat element allowed quantification of T. gondii DNA. The contamination level in different types of tissue and in two groups of goats euthanized 30 and 90 dpi was compared. The highest concentration of T. gondii DNA in both groups of goats was found in lung tissue, but only the higher parasite count in lung tissue compared to other organs in group A (euthanized 30 dpi) was statistically significant. T. gondii concentrations were higher in liver and dorsal muscle samples from goats euthanized 90 dpi than in goats euthanized at 30 dpi, while the T. gondii concentration in hearts decreased. This study describes for the first time distribution of T. gondii parasites in post-weaned goat kids. New information about T. gondii predilection sites in goats and about the progression of infection between 30 and 90 dpi was achieved.
未煮熟的含有组织包囊的肉是人感染刚地弓形虫最常见的来源之一。山羊对临床弓形虫病非常易感，尤其是儿童常被当作食物，因此代表了人类感染的风险。本研究采用序列特异性磁珠捕获法从实验感染山羊的组织样本中分离弓形虫 DNA，并采用 529 bp 重复元件的实时 PCR 定量 T. gondii DNA。比较了不同类型组织和两组在感染后 30 和 90 天被安乐死的山羊中的污染水平。在两组山羊中，弓形虫 DNA 的浓度在肺组织中最高，但仅在 A 组（感染后 30 天安乐死）的肺组织与其他器官相比寄生虫数量更高具有统计学意义。与感染后 30 天安乐死的山羊相比，感染后 90 天安乐死的山羊的肝和背肌样本中的弓形虫浓度更高，而心脏中的弓形虫浓度降低。本研究首次描述了刚地弓形虫在断奶后山羊幼崽中的分布。获得了有关山羊中弓形虫偏好部位以及在 30 至 90 天之间感染进展的新信息。
