新兴的时空控制干细胞命运和形态发生的策略。
Emerging strategies for spatiotemporal control of stem cell fate and morphogenesis.
机构信息
The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology/Emory University, Atlanta, GA, USA.
出版信息
Trends Biotechnol. 2013 Feb;31(2):78-84. doi: 10.1016/j.tibtech.2012.11.001. Epub 2012 Dec 5.
Abstract
Stem cell differentiation is regulated by the complex interplay of multiple parameters, including adhesive intercellular interactions, cytoskeletal and extracellular matrix remodeling, and gradients of agonists and antagonists that individually and collectively vary as a function of spatial locale and temporal stages of development. Current approaches to direct stem cell differentiation focus on systematically understanding the relative influences of microenvironmental perturbations and simultaneously engineering platforms aimed at recapitulating physicochemical aspects of tissue morphogenesis. This review focuses on novel approaches to control the spatiotemporal dynamics of stem cell signaling and morphogenic remodeling to direct the differentiation of stem cells and develop functional tissues for in vitro screening and regenerative medicine technologies.
摘要
干细胞分化受多种参数的复杂相互作用调控，包括细胞间黏附相互作用、细胞骨架和细胞外基质重塑，以及激动剂和拮抗剂的梯度，这些参数单独或集体随空间位置和发育的时空阶段而变化。目前，定向干细胞分化的方法主要集中在系统地理解微环境扰动的相对影响，并同时设计旨在再现组织形态发生的物理化学方面的工程平台。本综述重点介绍了控制干细胞信号和形态发生重塑时空动力学的新方法，以指导干细胞的分化，并为体外筛选和再生医学技术开发功能性组织。
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