Zevenhuizen L P, van Veldhuizen A, Fokkens R H
Laboratory of Microbiology, Agricultural University, Wageningen, The Netherlands.
Antonie Van Leeuwenhoek. 1990 Apr;57(3):173-8. doi: 10.1007/BF00403952.
Gel-filtration and thin layer chromatography of low molecular weight carbohydrates from culture filtrates of Agrobacterium radiobacter, Isolate II, have shown, that next to the neutral beta-1,2-glucan fraction a major acidic fraction was present which was found to be glycerophosphorylated cyclic beta-1,2-glucans. Re-examination of cyclic beta-1,2-glucan preparations which had been obtained by extraction of Rhizobium cells with hot phenol-water also showed these acidic modified beta-1,2-glucans to be present. Cyclic beta-1,2-glucans from R. leguminosarum (9 strains) and of R. phaseoli (1 strain) had ring size distribution with degrees of polymerisation (DPs) of 19 and 20 as major ring sizes of which a minor part was glycerophosphorylated; beta-1,2-glucans of R. trifolii (3 strains) had ring sizes with DPs measuring 19-22 as prominent components which were largely unsubstituted, and R. meliloti (7 strains) had beta-1,2-glucans with ring size distributions extending to still higher DPs of 19-25 of which the major part appeared to be glycerophosphorylated.
对放射土壤杆菌分离株II培养滤液中的低分子量碳水化合物进行凝胶过滤和薄层色谱分析表明，除了中性β-1,2-葡聚糖部分外，还存在一个主要的酸性部分，该部分被发现是甘油磷酸化的环状β-1,2-葡聚糖。对通过用热酚水提取根瘤菌细胞获得的环状β-1,2-葡聚糖制剂进行重新检查，也表明存在这些酸性修饰的β-1,2-葡聚糖。来自豆科根瘤菌（9个菌株）和菜豆根瘤菌（1个菌株）的环状β-1,2-葡聚糖的环大小分布中，聚合度（DPs）为19和20的环为主要环大小，其中一小部分是甘油磷酸化的；三叶草根瘤菌（3个菌株）的β-1,2-葡聚糖的环大小以DPs为19 - 22的环为主要成分，且大部分未被取代，而苜蓿根瘤菌（7个菌株）的β-1,2-葡聚糖的环大小分布延伸至更高的DPs，为19 - 25，其中大部分似乎是甘油磷酸化的。
