Zorreguieta A, Cavaignac S, Geremia R A, Ugalde R A
Instituto de Investigaciones Bioquimicas, Fundación Campomar, CONICET, Buenos Aires, Argentina.
J Bacteriol. 1990 Aug;172(8):4701-4. doi: 10.1128/jb.172.8.4701-4704.1990.
High osmolarity in the culture medium of growing Agrobacterium tumefaciens strongly inhibited the accumulation of cellular beta(1-2) glucan. However, the enzymatic system required for the synthesis of this polysaccharide from UDP-glucose was not repressed by high osmolarity. Mutants of A. tumefaciens and Rhizobium meliloti affected in beta(1-2) glucan synthesis were unable to grow normally in low-osmolarity media.
在生长的根癌土壤杆菌培养基中高渗透压强烈抑制细胞β(1-2)葡聚糖的积累。然而,由UDP-葡萄糖合成这种多糖所需的酶系统并未被高渗透压抑制。在β(1-2)葡聚糖合成中受影响的根癌土壤杆菌和苜蓿根瘤菌突变体在低渗透压培养基中无法正常生长。