Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, Republic of Korea.
Anal Biochem. 2013 Mar 1;434(1):199-201. doi: 10.1016/j.ab.2012.11.009. Epub 2012 Dec 3.
We report on a simple, fast, and highly sensitive method that allows femtomolar detection of microRNAs (miRNAs) without the need of polymerase chain reaction using a nanoengineered micro gold shell (μAuS) and laser desorption/ionization time-of-flight (LDI-TOF) mass spectrometry (MS). Target miRNAs are selectively captured by a chemically decorated gold chip and a μAuS, which carries with it a large number of small molecules, termed amplification tags (Am-tags). Subsequent LDI-TOF MS analysis allows a highly amplified mass signal of Am-tags for the presence of target miRNAs in a sample, resulting in ultrasensitive detection.
我们报告了一种简单、快速且高度灵敏的方法，该方法无需聚合酶链反应（PCR），使用纳米工程微金壳（μAuS）和激光解吸/电离飞行时间（LDI-TOF）质谱（MS）即可实现对微 RNA（miRNA）的飞摩尔检测。目标 miRNA 被化学修饰的金芯片和携带大量小分子的 μAuS 选择性捕获，这些小分子被称为扩增标签（Am-tags）。随后的 LDI-TOF MS 分析允许对 Am-tags 的放大质量信号进行高度放大，以检测样品中目标 miRNA 的存在，从而实现超灵敏检测。
