Ha Mi-Sun, Seo Hyunjung, Bae Dong-Ho, Yeo Woon-Seok
Department of Bioscience and Biotechnology, Konkuk University.
Anal Sci. 2014;30(4):451-5. doi: 10.2116/analsci.30.451.
This paper describes a new, simple, and sensitive method for detecting two fluoroquinolones: enrofloxacin and its metabolite ciprofloxacin, which are widely used as drugs for humans and animals. We utilized gold nanoparticles (AuNPs) and laser desorption/ionization time-of-flight (LDI-TOF) mass spectrometry (MS) with a matrix-free format. An antibody for the drug was immobilized on a chip based on self-assembled monolayers (SAMs) on gold, and AuNPs were decorated with the drug along with a large excess of small molecules, called amplification tags (Am-tags). In this strategy, target drugs in solution bound to the antibody on the chip compete with the AuNP-immobilized drugs. The presence of targets was verified by the amplified LDI-TOF MS signals of Am-tags on AuNPs.
本文描述了一种用于检测两种氟喹诺酮类药物的新的、简单且灵敏的方法：恩诺沙星及其代谢产物环丙沙星，这两种药物广泛用于人和动物。我们采用了金纳米颗粒（AuNPs）以及无基质形式的激光解吸/电离飞行时间（LDI-TOF）质谱（MS）。基于金表面的自组装单分子层（SAMs），将药物抗体固定在芯片上，并且用药物以及大量过量的小分子（称为扩增标签，Am-tags）修饰AuNPs。在该策略中，溶液中的目标药物与芯片上的抗体结合，与固定在AuNPs上的药物竞争。通过AuNPs上Am-tags的LDI-TOF MS信号放大来验证目标的存在。
