Department of Maxillofacial Surgery, Azahra Hospital, Isfahan University of Medical Sciences, Isfahan, Iran.
Int J Oral Maxillofac Surg. 2013 May;42(5):562-8. doi: 10.1016/j.ijom.2012.10.012. Epub 2012 Dec 7.
This study was designed to compare bone regeneration of tissue-engineered bone from adipose-derived stem cell and autogenous bone graft in a canine maxillary alveolar cleft model. In this prospective clinical trial, mesenchymal stem cells (MSCs) were isolated from subcutaneous canine adipose tissue. Undifferentiated cells were incubated with a 3mm×3mm×3mm hydroxyapatite/beta-tricalcium phosphate scaffold, in specific osteogenic medium for 21 days. Four mongrel dogs were prepared by removal of two of the three incisors bilaterally and a 15mm defect in bone was created from crest to nasal floor. After healing, repair was followed by a tissue engineered bone graft from adipose-derived stem cells on one side and corticocancellous tibial auto graft on the other side. Bone regeneration was evaluated by histomorphometry on days 15 and 60 after implantation. The data were analysed with descriptive and t test methods (α=0.05). Bone formation on the autograft sides was higher than on the stem cell sides at 15 and 60 days, 45% and 96% versus 5% and 70%, respectively. Differences between the two groups at 15 and 60 days were significant (p=0.004 and 0.001, respectively). Although autograft is still the gold standard for bone regeneration, tissue engineered bone may provide an acceptable alternative.
本研究旨在比较脂肪来源干细胞组织工程骨与自体骨移植物在犬上颌牙槽裂模型中的骨再生情况。在这项前瞻性临床试验中，从犬皮下脂肪组织中分离间充质干细胞（MSCs）。未分化细胞在特定的成骨培养基中与 3mm×3mm×3mm 的羟基磷灰石/β-磷酸三钙支架孵育 21 天。通过去除双侧三个切牙中的两个，并在嵴到鼻底处形成 15mm 的骨缺损，使 4 只杂种犬做好准备。愈合后，一侧采用脂肪来源干细胞组织工程骨，另一侧采用皮质松质胫骨自体移植物进行修复。在植入后 15 和 60 天通过组织形态计量学评估骨再生。使用描述性和 t 检验方法（α=0.05）分析数据。在 15 和 60 天时，自体移植物侧的骨形成高于干细胞侧，分别为 45%和 96%与 5%和 70%。两组在 15 和 60 天时的差异均具有统计学意义（p=0.004 和 0.001）。虽然自体移植物仍然是骨再生的金标准，但组织工程骨可能是一种可接受的替代方法。
