Dental Institute, Chinese PLA General Hospital, Beijing, China.
Clin Implant Dent Relat Res. 2013 Jun;15(3):390-401. doi: 10.1111/j.1708-8208.2011.00373.x. Epub 2011 Jul 11.
Lack of osseointegration between a dental implant and the walls of the alveolar bone is a common problem in immediate implantation. Injectable tissue-engineered bone (ITB) may be an effective and minimally invasive solution to the problem. In this study, an injectable bone cement, nHAC/CSH, which consists of nano-hydroxyapatite/collagen (nHAC) and calcium sulfate hemihydrate (CaSO4 .½H2 O; CSH) was investigated as a tissue-engineered scaffold material with blood-acquired mesenchymal progenitor cells (BMPC) as seeding cells.
The aim of the study was to assess the new bone formation around immediate dental implants using nHAC/CSH loaded with dog blood-acquired mesenchymal progenitor cells (dBMPC) in a canine model.
dBMPC were first isolated from peripheral blood of healthy adult dogs. Alizarin red and oil red O staining were then used to evaluate the potential of dBMPC to differentiate into bi-lineage mesenchymal tissues in vitro. Four healthy mongrel dogs were used in this study. The alveolar bone defects around immediate implants of dogs were created. Each defect was randomly assigned to one of the following three groups: (1) the ITB group (dBMPC+nHAC/CSH); (2) injectable bone cement nHAC/CSH; or (3) no materials (controls). Methylene blue staining was used to examine the bone formation after 3 months.
Studies in vitro revealed that dBMPC could be induced to osteoblasts and adipocytes. The ITB group (dBMPC+nHAC/CSH) showed significantly more bone-implant contact and bone density than either nHAC/CSH or control groups in the areas with peri-implant defects 3 months after implantation.
The results indicate that the ITB composed of nHAC/CSH and dBMPC may represent a useful strategy for the clinical reconstruction of bone defects around immediate implantation. However, further investigation is needed involving the use of human BMPC as well as possible use of stem cells.
种植体与牙槽骨之间缺乏骨整合是即刻种植的常见问题。可注射组织工程骨（ITB）可能是解决这一问题的有效且微创的方法。在这项研究中，我们将由纳米羟基磷灰石/胶原（nHAC）和半水硫酸钙（CaSO4.½H2 O；CSH）组成的可注射骨水泥 nHAC/CSH 作为组织工程支架材料进行研究，以犬血液获得的间充质祖细胞（BMPC）作为种子细胞。
本研究旨在通过犬模型，评估负载犬血液获得的间充质祖细胞（dBMPC）的 nHAC/CSH 对即刻种植体周围新骨形成的影响。
首先从健康成年犬外周血中分离 dBMPC。茜素红和油红 O 染色用于评估 dBMPC 在体外向双谱系间充质组织分化的潜能。本研究使用了 4 只健康杂种犬。在犬即刻种植体周围的牙槽骨缺损处，分别将 ITB 组（dBMPC+nHAC/CSH）、可注射骨水泥 nHAC/CSH 组或无材料对照组（空白对照组）随机分配至其中一个组。3 个月后通过亚甲蓝染色观察骨形成情况。
体外研究显示，dBMPC 可诱导为成骨细胞和脂肪细胞。植入后 3 个月，在种植体周围缺损区域，ITB 组（dBMPC+nHAC/CSH）的骨-种植体接触率和骨密度均显著高于 nHAC/CSH 组和空白对照组。
结果表明，由 nHAC/CSH 和 dBMPC 组成的 ITB 可能为即刻种植周围骨缺损的临床重建提供一种有用的策略。然而，还需要进一步研究，包括使用人 BMPC 以及可能使用干细胞。
