含间充质基质细胞的组织工程骨移植物用于大鼠牙槽裂骨成形术的疗效
Efficacy of tissue engineered bone grafts containing mesenchymal stromal cells for cleft alveolar osteoplasty in a rat model.
作者信息
Korn P, Schulz M C, Range U, Lauer G, Pradel W
机构信息
Department of Oral and Maxillofacial Surgery (Head: Prof. Dr. med. Dr. med. dent. Günter Lauer), Faculty of Medicine "Carl Gustav Carus", Technische Universität Dresden, Fetscherstr. 74, D-01307 Dresden, Germany.
Department of Oral and Maxillofacial Surgery (Head: Prof. Dr. med. Dr. med. dent. Günter Lauer), Faculty of Medicine "Carl Gustav Carus", Technische Universität Dresden, Fetscherstr. 74, D-01307 Dresden, Germany.
出版信息
J Craniomaxillofac Surg. 2014 Oct;42(7):1277-85. doi: 10.1016/j.jcms.2014.03.010. Epub 2014 Apr 4.
UNLABELLED
The development of sufficient tissue engineered bone grafts for alveolar cleft osteoplasty could reduce the necessity of autogenous bone grafts and its donor site morbidity. The aim of the study was to evaluate tissue engineered bone grafts in an artificially created bone defect. Bone grafts were created in vitro colonizing a synthetic hydroxyapatite-tricalciumphosphate scaffold (BONITmatrix(®)) with either undifferentiated mesenchymal stromal cells (group 1) or osteogenic differentiated mesenchymal stromal cells (group 2). Cells were multiplied from bone marrow of donor rats. Unmodified scaffolds (group 3) and the tissue engineered bone grafts were inserted into artificial maxillary defects of 54 Lewis rats. In 18 animals the defects remained unfilled (control). After one, three and six weeks the rats were sacrificed. The defect was evaluated radiologically and histologically with regard to the remaining defect volume and diameter. Statistical analysis followed. The bone grafts led to a specific bone formation at the defect margin. No complete reunion of any defect was observed within the healing time. After six weeks, the remaining defect volume was 6.86 ± 3.21 mm(3) (control), 4.08 ± 1.36 mm(3) (group 1), 5.00 ± 0.84 mm(3) (group 2) 5.50 ± 1.05 mm(3) (group 3). The remaining defect diameter measured 2.63 ± 0.52 mm (control), 2.39 ± 0.23 mm (group 1), 2.53 ± 0.22 mm (group 2) and 2.70 ± 0.66 mm (group 3). In all experimental groups the defect volume and diameter decreased over time, which was significant for group 1 (p = 0.014), group 2 (p = 0.025) and group 3 (p = 0.048). The defect volume and width was significantly reduced for bone grafts containing undifferentiated cells compared to control (p = 0.035) or scaffolds only (p = 0.05).
CONCLUSION
Tissue engineered bone grafts induce a pronounced bone formation in artificial bone defects compared to unfilled controls or scaffolds only.
未标注
开发足够的组织工程骨移植物用于牙槽裂骨成形术可减少自体骨移植的必要性及其供区并发症。本研究的目的是评估在人工制造的骨缺损中组织工程骨移植物的效果。通过用未分化的间充质基质细胞（第1组）或成骨分化的间充质基质细胞（第2组）定植合成羟基磷灰石 - 磷酸三钙支架（BONITmatrix(®)）在体外制造骨移植物。细胞从供体大鼠的骨髓中增殖。将未修饰的支架（第3组）和组织工程骨移植物植入54只Lewis大鼠的人工上颌骨缺损中。18只动物的缺损保持未填充状态（对照组）。在1、3和6周后处死大鼠。对缺损进行放射学和组织学评估，观察剩余缺损体积和直径。随后进行统计分析。骨移植物在缺损边缘导致了特定的骨形成。在愈合时间内未观察到任何缺损完全愈合。6周后，剩余缺损体积分别为6.86±3.21mm³（对照组）、4.08±1.36mm³（第1组）、5.00±0.84mm³（第2组）、5.50±1.05mm³（第3组）。剩余缺损直径分别为2.63±0.52mm（对照组）、2.39±0.23mm（第1组）、2.53±0.22mm（第2组）和2.70±0.66mm（第3组）。在所有实验组中，缺损体积和直径随时间减小，第1组（p = 0.014）、第2组（p = 0.025）和第3组（p = 0.048）差异有统计学意义。与对照组（p = 0.035）或仅支架组（p = 0.05）相比，含有未分化细胞的骨移植物的缺损体积和宽度显著减小。
结论
与未填充的对照组或仅支架组相比，组织工程骨移植物在人工骨缺损中诱导明显的骨形成。
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