Li Fei, Yang Xiaolin, Bi Jianping, Yang Zhonglin, Zhang Chunfeng
a State Key Laboratory of Natural Medicines , China Pharmaceutical University , Nanjing , PR China ;
b Key Laboratory of Pharmaceutical and Biological Marine Resources Research and Development of Jiangsu Province , Nanjing University of Chinese Medicine , Nanjing , PR China ;
Pharm Biol. 2016 Sep;54(9):1857-64. doi: 10.3109/13880209.2015.1133657. Epub 2016 Jan 13.
Context Eucommiae Cortex and Radix Dipsaci, occurring in a ratio of 1:1 in Du-Zhong-Wan (DZW), a Chinese herbal medicine, is available as a water extract followed by ethanol precipitation for the treatment of osteoporosis, fractures and menopausal syndrome. Objective This study investigates the protective effects of DZW in ovariectomy (OVX)-induced bone loss in a rat osteopenia model. Materials and methods Sixty Sprague-Dawley rats were randomly divided into the sham-operated group (SHAM) and five OVX subgroups: OVX with vehicle (OVX), 17β-estradiol (E2) and with three graded doses of DZW. Daily oral administration of the different samples started on the fifth week and lasted for 12 weeks, respectively. The body weight, uterus wet weight, serum biochemical parameters, bone mineral density (BMD), bone biomechanical properties, bone microarchitecture and immunohistochemistry were examined. Results Compared with the SHAM group, the DZW treatment significantly reversed the osteoporotic changes in OVX rats. The DZW-H group showed that serum tartrate-resistant acid phosphatase 5b (TRACP-5b) levels reduced by 152.25% (p < 0.01) and osteocalein (OCN) levels dose dependently increased by 118.43% (p < 0.01) as compared with the OVX group. Compared with the OVX group, the DZW at different three dosages of DZW evidently increased the right femur BMD by 112.43, 114.56 and 116.45%, and dramatically promoted bone quality and bone strength (p < 0.05). Further, immunohistochemical evaluation also showed that DZW administration increased ER expression in uteri (p < 0.01). Conclusions DZW exhibits an anti-osteoporotic effect, probably mediated via phyto-estrogenic effects. It might be a potential herbal alternative for the management of postmenopausal osteoporosis.
杜仲皮和续断在中药杜仲丸（DZW）中按1:1的比例存在，其水提取物经乙醇沉淀后可用于治疗骨质疏松症、骨折和更年期综合征。
本研究在大鼠骨质疏松模型中研究杜仲丸对去卵巢（OVX）诱导的骨质流失的保护作用。
60只Sprague-Dawley大鼠随机分为假手术组（SHAM）和五个去卵巢亚组：去卵巢对照组（OVX）、17β-雌二醇（E2）组以及三个不同剂量的杜仲丸组。从第五周开始每日口服不同样品，持续12周。检测体重、子宫湿重、血清生化参数、骨密度（BMD）、骨生物力学性能、骨微结构和免疫组化。
与SHAM组相比，杜仲丸治疗显著逆转了OVX大鼠的骨质疏松变化。与OVX组相比，杜仲丸高剂量组（DZW-H）血清抗酒石酸酸性磷酸酶5b（TRACP-5b）水平降低了152.25%（p<0.01），骨钙素（OCN）水平剂量依赖性增加了118.43%（p<0.01）。与OVX组相比，三种不同剂量的杜仲丸明显使右侧股骨骨密度分别增加了112.43%、114.56%和116.45%，并显著改善了骨质量和骨强度（p<0.05）。此外，免疫组化评估还显示，给予杜仲丸可增加子宫中雌激素受体（ER）的表达（p<0.01）。
杜仲丸具有抗骨质疏松作用，可能是通过植物雌激素效应介导的。它可能是治疗绝经后骨质疏松症的一种潜在的草药替代品。
