基于毛细管电泳的多重 PCR 检测法在鉴定念珠菌属种特异性中的应用
Usefulness of capillary electrophoresis-based multiplex PCR assay for species-specific identification of Candida spp.
机构信息
Department of Biomedical Sciences, Microbiology and Virology Unit, University of Cagliari, Italy.
出版信息
J Microbiol Methods. 2013 Feb 15;92(2):150-2. doi: 10.1016/j.mimet.2012.11.022. Epub 2012 Dec 7.
The study evaluated the performances of a commercial multiplex PCR assay, the Seegene Seeplex STI Master Panel 3, for Candida spp. identification. Eighty clinical strains of Candida spp. were identified with this system and a homemade multiplex PCR assay. The results were also compared with those obtained with two phenotypic methods. The study provided a preliminary evaluation of a multiplex assay from Seegene that uses capillary electrophoresis as the detection of amplified products. The Seeplex assay was found to be a rapid and useful method for identifying large numbers of yeast isolates in the clinical laboratory context.
本研究评估了一种商业多重 PCR 检测试剂盒，即 Seegene 公司的 Seeplex STI Master Panel 3，用于鉴定念珠菌属。该系统和一种自制的多重 PCR 检测方法共鉴定了 80 株念珠菌属临床分离株。结果还与两种表型方法的结果进行了比较。本研究初步评估了一种基于毛细管电泳检测扩增产物的 Seegene 多重检测方法。该方法快速且有助于临床实验室鉴定大量酵母分离株。
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