基于毛细管凝胶电泳的多重 PCR 检测与核糖体基因间转录间隔区 2 扩增测序在鉴定临床重要念珠菌种中的比较。

Comparison of a capillary gel electrophoresis-based multiplex PCR assay and ribosomal intergenic transcribed spacer-2 amplicon sequencing for identification of clinically important Candida species.

机构信息

Division of Clinical Microbiology, Department of Experimental Medicine, Faculty of Health Sciences, Linköping University, County Council of Östergötland, S-581 85 Linköping, Sweden.

Division of Clinical Microbiology, Department of Experimental Medicine, Faculty of Health Sciences, Linköping University, County Council of Östergötland, S-581 85 Linköping, Sweden.

出版信息

J Microbiol Methods. 2014 Jan;96:81-3. doi: 10.1016/j.mimet.2013.11.004. Epub 2013 Nov 16.

DOI:10.1016/j.mimet.2013.11.004

Abstract

The performance of a commercially available Seegene Seeplex STI Master Panel 3 multiplex PCR for Candida species identification was compared with an internal transcribed spacer 2 (ITS2) PCR assay. We found that the Seeplex assay was specific for identification of C. albicans, C. krusei, C. parapsilosis, C. glabrata, C. tropicalis and C. dubliniensis.

摘要

我们将商用的 Seegene Seeplex STI Master Panel 3 多重 PCR 用于念珠菌属种鉴定的性能与内部转录间隔区 2（ITS2）PCR 检测进行了比较。我们发现 Seeplex 检测法可特异性鉴定白念珠菌、克柔念珠菌、近平滑念珠菌、光滑念珠菌、热带念珠菌和都柏林念珠菌。

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PLoS One. 2016 Apr 22;11(4):e0154385. doi: 10.1371/journal.pone.0154385. eCollection 2016.

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