Department of Pathology, St. Jude Children's Research Hospital , Memphis, TN, USA.
Med Mycol. 2012 Apr;50(3):259-65. doi: 10.3109/13693786.2011.600341. Epub 2011 Aug 23.
The application of molecular diagnostic methods may improve the timeliness and accuracy with which fungi are identified. A total of 76 well-characterized reference strains of clinically relevant Candida species and 61 clinical Candida isolates were tested by repetitive sequence PCR (rep-PCR) and PCR followed by electrospray ionization mass spectrometry (PCR/ESI-MS) and results compared against internal transcribed spacer (ITS) ribosomal RNA gene sequencing as a reference standard. Both rep-PCR and PCR/ESI-MS correctly identified 51 isolates to the species level. When method performance was evaluated based only on genospecies included in the reference libraries, both methods yielded an accuracy of 98.1%. It may be concluded that rep-PCR and PCR/ESI-MS are highly effective at identifying clinical isolates of Candida to the species level. These methods hold promise for improving the speed and accuracy of identification of Candida spp. in clinical mycology laboratories.
分子诊断方法的应用可以提高真菌鉴定的及时性和准确性。通过重复序列 PCR（rep-PCR）和聚合酶链反应（PCR）后电喷雾电离质谱（PCR/ESI-MS），对 76 株临床相关念珠菌种的特征明确的参考菌株和 61 株临床念珠菌分离株进行了检测，并与内部转录间隔区（ITS）核糖体 RNA 基因测序作为参考标准进行了比较。rep-PCR 和 PCR/ESI-MS 均正确地将 51 株分离株鉴定到种水平。当仅根据参考文库中包含的基因种评估方法性能时，两种方法的准确性均为 98.1%。可以得出结论，rep-PCR 和 PCR/ESI-MS 非常有效地将临床分离株鉴定为念珠菌种。这些方法有望提高临床真菌学实验室中念珠菌属鉴定的速度和准确性。
