利用环介导等温扩增技术快速灵敏检测鲍曼不动杆菌。
Rapid and sensitive detection of Acinetobacter baumannii using loop-mediated isothermal amplification.
机构信息
Department of Laboratory Medicine and Biotechnology, Tzu Chi University, Hualien, Taiwan.
出版信息
J Microbiol Methods. 2013 Feb 15;92(2):197-200. doi: 10.1016/j.mimet.2012.11.020. Epub 2012 Dec 5.
PMID:23220188
Abstract
Here we report the design and evaluation of a loop-mediated isothermal amplification (LAMP) assay for detecting Acinetobacter baumannii DNA based on the 16S-23S rRNA intergenic spacer (ITS) sequence. The results showed that target DNA was amplified and visualized within 30min and with a detection limit 100-fold greater than PCR.
摘要
我们在此报告了一种基于 16S-23S rRNA 基因间隔区（ITS）序列的环介导等温扩增（LAMP）检测鲍曼不动杆菌 DNA 的设计和评估。结果表明，目标 DNA 在 30 分钟内得到扩增和可视化，检测限比 PCR 高 100 倍。
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