Vijayakumar Saranya, Biswas Indranil, Veeraraghavan Balaji
Department of Clinical Microbiology, Christian Medical College, Vellore 632 004, Tamil Nadu, India.
Department of Microbiology, Molecular Genetics & Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA.
Future Sci OA. 2019 Jun 27;5(6):FSO395. doi: 10.2144/fsoa-2018-0127.
species have emerged as one of the most clinically important pathogens. The phenotypic techniques which are currently available are insufficient in accurately identifying and differentiating the closely related and clinically important species. Here, we discuss the advantages and limitations of the conventional phenotypic methods, automated identification systems, molecular methods and MALDI-TOF in the precise identification and differentiation of species. More specifically, several species of this genus are increasingly reported to be of high clinical importance. Molecular characterization such as of -like PCR together with sequencing has high discriminatory power over the conventional methods for species identification, especially within the complex.
该菌种已成为临床上最重要的病原体之一。目前可用的表型技术不足以准确识别和区分密切相关且具有临床重要性的菌种。在此，我们讨论传统表型方法、自动化鉴定系统、分子方法和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）在该菌种精确鉴定和区分方面的优势与局限性。更具体地说，该属的几个菌种越来越多地被报道具有高度临床重要性。诸如类PCR结合测序等分子特征分析方法在该菌种鉴定方面比传统方法具有更高的鉴别力，尤其是在该菌种复合体内部。
