Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80303, USA.
J Biol Chem. 2013 Jan 11;288(2):1420-7. doi: 10.1074/jbc.X112.442855. Epub 2012 Dec 6.
It is a great privilege to contribute to the Reflections essays. In my particular case, this essay has allowed me to weave some of my major scientific contributions into a tapestry held together by what I have learned from three colleagues (Robert Letsinger, Gobind Khorana, and George Rathmann) who molded my career at every important junction. To these individuals, I remain eternally grateful, as they always led by example and showed many of us how to break new ground in both science and biotechnology. Relative to my scientific career, I have focused primarily on two related areas. The first is methodologies we developed for chemically synthesizing DNA and RNA. Synthetic DNA and RNA continue to be an essential research tool for biologists, biochemists, and molecular biologists. The second is developing new approaches for solving important biological problems using synthetic DNA, RNA, and their analogs.
非常荣幸能为《反思》系列文章撰稿。在我个人的案例中，本文使我得以将一些重要的科学贡献编织在一起，这些贡献通过我从三位同事（罗伯特·莱辛格、戈宾·霍拉纳和乔治·拉思曼）那里学到的东西串联起来，他们在职业生涯的每一个重要转折点塑造了我的事业。我对这些人永远心怀感激，因为他们以身作则，向我们许多人展示了如何在科学和生物技术领域开辟新天地。相对于我的科学事业，我主要关注两个相关领域。第一个是我们开发的用于化学合成 DNA 和 RNA 的方法。合成 DNA 和 RNA 仍然是生物学家、生物化学家以及分子生物学家不可或缺的研究工具。第二个是利用合成 DNA、RNA 及其类似物开发解决重要生物学问题的新方法。
