Divisions of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA.
Clin Infect Dis. 2013 Apr;56(7):996-1002. doi: 10.1093/cid/cis1014. Epub 2012 Dec 7.
In the first decade of the 21st century, we have seen the completion of the human genome project and marked progress in the human microbiome project. The vast amount of data generated from these efforts combined with advances in molecular and biomedical technologies have led to the development of a multitude of assays and technologies that may be useful in the diagnosis and management of infectious diseases. Here, we identify several new assays and technologies that have recently come into clinical use or have potential for clinical use in the near future. The scope of this review is broad and includes topics such as the serum marker procalcitonin, gene expression profiling, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and nucleic acid aptamers. Principles that underlie each assay or technology, their clinical applications, and potential strengths and limitations are addressed.
在 21 世纪的第一个十年里，我们见证了人类基因组计划的完成和人类微生物组计划的显著进展。这些努力产生的大量数据，结合分子和生物医学技术的进步，催生了许多可能有助于传染病诊断和管理的检测方法和技术。在这里，我们确定了几种最近已投入临床使用或在不久的将来具有临床应用潜力的新检测方法和技术。本综述的范围很广，包括血清标志物降钙素原、基因表达谱分析、基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）和核酸适体等主题。本文介绍了每种检测方法或技术的基本原理、临床应用以及潜在的优势和局限性。
