CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, Anhui 230026, P. R. China.
Chem Asian J. 2013 Feb;8(2):385-91. doi: 10.1002/asia.201201033. Epub 2012 Dec 7.
Multimodal imaging and simultaneous therapy is highly desirable because it can provide complementary information from each imaging modality for accurate diagnosis and, at the same time, afford an imaging-guided focused tumor therapy. In this study, indocyanine green (ICG), a near-infrared (NIR) imaging agent and perfect NIR light absorber for laser-mediated photothermal therapy, was successfully incorporated into superparamagnetic Fe(3)O(4)@mSiO(2) core-shell nanoparticles to combine the merit of NIR/magnetic resonance (MR) bimodal imaging properties with NIR photothermal therapy. The resultant nanoparticles were homogenously coated with poly(allylamine hydrochloride) (PAH) to make the surface of the composite nanoparticles positively charged, which would enhance cellular uptake driven by electrostatic interactions between the positive surface of the nanoparticles and the negative surface of the cancer cell. A high biocompatibility of the achieved nanoparticles was demonstrated by using a cell cytotoxicity assay. Moreover, confocal laser scanning microscopy (CLSM) observations indicated excellent NIR fluorescent imaging properties of the ICG-loaded nanoparticles. The relatively high r(2) value (171.6 mM(-1) s(-1)) of the nanoparticles implies its excellent capability as a contrast agent for MRI. More importantly, the ICG-loaded nanoparticles showed perfect NIR photothermal therapy properties, thus indicating their potential for simultaneous cancer diagnosis as highly effective NIR/MR bimodal imaging probes and for NIR photothermal therapy of cancerous cells.
多模态成像和同时治疗是非常理想的，因为它可以提供来自每个成像模式的互补信息，用于准确的诊断，同时提供成像引导的聚焦肿瘤治疗。在这项研究中，吲哚菁绿（ICG），一种近红外（NIR）成像剂和激光介导光热疗法的理想近红外光吸收剂，成功地被掺入超顺磁 Fe（3）O（4）@mSiO（2）核壳纳米粒子中，将 NIR/磁共振（MR）双模态成像特性与 NIR 光热治疗结合起来。所得的纳米粒子被均匀地涂覆有聚（烯丙基盐酸盐）（PAH），使复合纳米粒子的表面带正电荷，这将增强纳米粒子的正表面与癌细胞的负表面之间的静电相互作用所驱动的细胞摄取。通过细胞毒性测定证明了所获得的纳米粒子具有高生物相容性。此外，共焦激光扫描显微镜（CLSM）观察表明，载有 ICG 的纳米粒子具有出色的近红外荧光成像性能。纳米粒子的相对高 r（2）值（171.6 mM（-1）s（-1））表明其作为 MRI 造影剂的优异性能。更重要的是，载有 ICG 的纳米粒子表现出完美的近红外光热治疗性能，因此表明其作为高效 NIR/MR 双模态成像探针用于癌症诊断和用于癌细胞的 NIR 光热治疗的潜力。
