Zahran Asmaa Mohamed, Elsayh Khalid Ibrahem, Metwalley Kotb Abbass
Department of Clinical Pathology, South Egypt Cancer Institute, Egypt.
Indian J Endocrinol Metab. 2012 Nov;16(6):952-7. doi: 10.4103/2230-8210.102998.
Regulatory T cells have an important role in the control of immune reactivity against self antigens and probably play a role in pathogenesis of type 1 diabetes (T1D). We aimed to determine the frequency of regulatory T cells in recently diagnosed children with/T1D.
20 children with/T1D and 20 healthy children of matched age and sex as controls were enrolled in this study. All cases were subjected to a thorough history taking, full clinical examinations and investigations which include; insulin C peptide levels and flow cytometric detection of B-, T-lymphocytes and regulatory T cells.
Insulin C peptide level was significantly lower in children with/ T1D compared with controls. The percentages of B and T-lymphocytes were not significantly different between patients and controls. The percentages of CD4+CD25+High and CD4+CD25+High Foxp3+ cells both in total lymphocytes and in CD4+ lymphocytes were significantly decreased in patients than controls, while the percentages of total CD4+CD25+ and CD4+CD25+Intermediate both in total lymphocytes and in CD4+ lymphocytes were not significantly different between patients and controls. The geometric mean of fluorescence intensity (MFI) of Foxp3+ expression in CD4+CD25+High cells was significantly decreased in patients than controls. Positive correlations were observed between both age and insulin C peptide and frequency of CD4+CD25+High Foxp3.
The percentage of regulatory T cells; CD4+CD25+High Foxp3 was decreased in children with recent T1D and may have a role in its pathogenesis. Their role as a prognostic signifi cance and their relation to various complications should be explored.
调节性T细胞在控制针对自身抗原的免疫反应中起重要作用，可能在1型糖尿病（T1D）的发病机制中发挥作用。我们旨在确定近期诊断为T1D的儿童中调节性T细胞的频率。
本研究纳入了20例T1D患儿和20例年龄及性别匹配的健康儿童作为对照。所有病例均进行了详细的病史采集、全面的临床检查和调查，包括胰岛素C肽水平以及B、T淋巴细胞和调节性T细胞的流式细胞术检测。
与对照组相比，T1D患儿的胰岛素C肽水平显著降低。患者和对照组之间B淋巴细胞和T淋巴细胞的百分比无显著差异。患者总淋巴细胞和CD4 +淋巴细胞中CD4 + CD25 + High和CD4 + CD25 + High Foxp3 +细胞的百分比均显著低于对照组，而患者和对照组总淋巴细胞和CD4 +淋巴细胞中总CD4 + CD25 +和CD4 + CD25 + Intermediate的百分比无显著差异。患者CD4 + CD25 + High细胞中Foxp3 +表达的荧光强度几何平均值（MFI）显著低于对照组。年龄和胰岛素C肽与CD4 + CD25 + High Foxp3频率之间均呈正相关。
近期患T1D的儿童中调节性T细胞（CD4 + CD25 + High Foxp3）的百分比降低，可能在其发病机制中起作用。应探讨它们作为预后指标的意义及其与各种并发症的关系。
