1Department of Oncological Clinical Pathology, South Egypt Cancer Institute, Assiut University, Assiut, Egypt.
Clin Appl Thromb Hemost. 2014 Jan;20(1):61-7. doi: 10.1177/1076029612454937. Epub 2012 Aug 7.
We aimed to examine the levels of lymphocyte subsets and regulatory T cells in patients with newly diagnosed immune thrombocytopenia (ITP) and their correlation with the course of ITP. The study included 40 pediatric patients with acute ITP and 30 controls. Lymphocytes and regulatory T cells were analyzed by flow cytometry. The percentages of CD19(+) and CD8(+) cells were significantly increased while that of CD4(+) cells and CD4(+)/CD8(+) ratio were significantly decreased. The percentages of CD4(+)CD25(+High) and CD4(+)CD25(+High) forkhead box protein 3 (Foxp3(+)) cells and the expression of Foxp3(+) in CD4(+)CD25(+High) cells were significantly decreased in patients. Age, platelet count, and mean platelet volume (MPV) in patients with brief duration of thrombocytopenia were significantly decreased than in those with prolonged duration. The percentages of CD8(+), CD4(+)CD25(+High), and CD4(+)CD25(+High) Foxp3(+) were significantly increased in patients with brief duration. Age, platelet count and MPV, and CD8+ cells had prognostic significance. CD4(+)CD25(+High) Foxp(+) T cells may be a helpful prognostic marker in children with acute ITP.
我们旨在研究新诊断的免疫性血小板减少症（ITP）患者淋巴细胞亚群和调节性 T 细胞的水平及其与 ITP 病程的相关性。该研究纳入了 40 例急性 ITP 患儿和 30 例对照。通过流式细胞术分析淋巴细胞和调节性 T 细胞。CD19(+)和 CD8(+)细胞的百分比显著升高，而 CD4(+)细胞和 CD4(+)/CD8(+)比值显著降低。CD4(+)CD25(+High)和 CD4(+)CD25(+High)叉头框蛋白 3（Foxp3(+)）细胞的百分比和 CD4(+)CD25(+High)细胞中 Foxp3(+)的表达显著降低。血小板减少持续时间短的患者的年龄、血小板计数和平均血小板体积（MPV）显著低于血小板减少持续时间长的患者。血小板减少持续时间短的患者 CD8(+)、CD4(+)CD25(+High)和 CD4(+)CD25(+High)Foxp3(+)的百分比显著升高。年龄、血小板计数和 MPV、以及 CD8+细胞具有预后意义。CD4(+)CD25(+High)Foxp(+)T 细胞可能是急性 ITP 患儿的一个有用的预后标志物。
