Franiek Natalie, Orth Dorothea, Grif Katharina, Ewers Christa, Wieler Lothar H, Thalhammer Johann G, Würzner Reinhard
Klinisches Department für Kleintiere und Pferde, Veterinärmedizinische Universität Wien.
Berl Munch Tierarztl Wochenschr. 2012 Nov-Dec;125(11-12):469-75.
In contrast to infections with enterohaemorrhagic E. coli (EHEC), which are thought to be classical zoonosis, the zoonotic potential of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae is still widely unknown. The aim of our study was to determine the frequency of EHEC and ESBL-producing Enterobacteriaceae in domestic animals (dogs and cats) in the Tyrol. Among 228 fecal samples of dogs (n = 92) and cats (n = 136) three samples (1.3%) were positive in the EHEC-ELISA. In two of the three cases isolation of the organism was not possible, the third sample of a two-year-old crossbreed bitch yielded EHEC O103:H2. In twelve of 228 (5.3%) fecal samples 13 ESBL-producing Enterobacteriaceae (in ten cats and two dogs) were found.These animals mainly derived from homes for animals (ten animals, 83%). 75% of the isolates belonged to the CTX-M-1-group, 8% to the CTX-M-2-group and 17% to the CTX-M-9-group. One isolate was positive for CTX-M-1 and CTX-M-9. Typing of the 13 ESBL-producing isolates by multilocus sequence typing (MLST) showed ten different sequence types, which points out the importance of the horizontal transfer of mainly plasmid-coded ESBL genes. Transmission of EHEC and ESBL-producing Enterobacteriaceae from domestic animals to humans is possible, corroborated by the fact that the EHEC serotype found in one dog and the sequence types detected by MLST in several dogs and cats were previously reported to occur in severe human infection.
与被认为是典型人畜共患病的肠出血性大肠杆菌（EHEC）感染不同，产超广谱β-内酰胺酶（ESBL）肠杆菌科细菌的人畜共患病潜力仍广为人知。我们研究的目的是确定蒂罗尔州家畜（狗和猫）中EHEC和产ESBL肠杆菌科细菌的频率。在228份狗（n = 92）和猫（n = 136）的粪便样本中，三份样本（1.3%）在EHEC酶联免疫吸附测定中呈阳性。在这三个案例中的两个案例中无法分离出该微生物，第三份样本来自一只两岁的杂种母犬，分离出EHEC O103:H2。在228份粪便样本中的12份（5.3%）中，发现了13株产ESBL肠杆菌科细菌（10只猫和2只狗）。这些动物主要来自动物收容所（10只动物，83%）。75%的分离株属于CTX-M-1组，8%属于CTX-M-2组，17%属于CTX-M-9组。一株分离株对CTX-M-1和CTX-M-9呈阳性。通过多位点序列分型（MLST）对13株产ESBL分离株进行分型，显示出十种不同的序列类型，这指出了主要由质粒编码的ESBL基因水平转移的重要性。EHEC和产ESBL肠杆菌科细菌从家畜传播给人类是可能的，一只狗中发现的EHEC血清型以及几只狗和猫中通过MLST检测到的序列类型先前已报道在严重人类感染中出现，这一事实证实了这一点。
