Laboratory of Veterinary Microbiology, Nippon Veterinary and Life Science University, Musashino, Tokyo, Japan.
Microbiol Immunol. 2012 Jul;56(7):480-5. doi: 10.1111/j.1348-0421.2012.00463.x.
Thirty-three cefazolin-resistant extraintestinal pathogenic Escherichia coli strains from companion animals were screened for bla(CMY-1) , bla(CMY-2) , bla(SHV) , bla(TEM) , and bla(CTX-M) genes. Extended-spectrum β-lactamase-producing strains were further characterized by O serotyping and multilocus sequence typing. It was found that 20 and 17 isolates harbored TEM-1 and CMY-2 β-lactamases, respectively, and 13 isolates harbored both β-lactamases. One isolate harbored DHA-1 β-lactamase. Eleven isolates were found to possess CTX-M β-lactamases (CTX-M-27 [n= 6], CTX-M-14 [n= 3], CTX-M-15 [n= 1], and CTX-M-55 [n= 1]). Of 11 CTX-M-positive strains, four strains were O25b-ST131 clones harboring CTX-M-27, and the remaining seven strains belonged to O6-ST127, ONT-ST354, O159-ST539, O1-ST648, O8-ST1642, O25b-ST2042, and ONT-ST2178.
从宠物动物中筛选了 33 株头孢唑啉耐药的肠外致病性大肠杆菌，以筛选 bla(CMY-1)、bla(CMY-2)、bla(SHV)、bla(TEM)和 bla(CTX-M)基因。对产超广谱β-内酰胺酶的菌株进一步进行 O 血清型和多位点序列分型。结果发现，20 株和 17 株分离株分别携带 TEM-1 和 CMY-2β-内酰胺酶，13 株分离株同时携带这两种β-内酰胺酶。1 株分离株携带 DHA-1β-内酰胺酶。发现 11 株分离株携带 CTX-Mβ-内酰胺酶（CTX-M-27[n=6]、CTX-M-14[n=3]、CTX-M-15[n=1]和 CTX-M-55[n=1]）。在 11 株 CTX-M 阳性菌株中，有 4 株 O25b-ST131 克隆株携带 CTX-M-27，其余 7 株分别属于 O6-ST127、ONT-ST354、O159-ST539、O1-ST648、O8-ST1642、O25b-ST2042 和 ONT-ST2178。
