Department of Histology and Anatomy, College of Oriental Medicine, Daegu Haany University, Gyeongsan 712-715, Republic of Korea.
Am J Chin Med. 2012;40(6):1241-55. doi: 10.1142/S0192415X12500929.
This study was performed to investigate effects of Curculigo orchioides rhizome (curculiginis rhizome) on acute reflux esophigitis (RE) in rats that are induced by pylorus and forestomach ligation operation. Proinflammatory cytokine, as well as tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6 were all assayed and the expression of TNF-α and COX2 analyzed by RT-PCR. The esophagic tissue damage of reflux esophagitis rat was increased compared to that of normal intact group. However, the esophagic damage percentage from the extract of curculiginis rhizoma (ECR) 600 mg/kg and ECR 300 mg/kg were significantly lower than that of the RE control group. Administration of α-tocopherol (30 mg/kg) and ECR (600 mg/kg, 300 mg/kg, and 150 mg/kg) had a significant effect on the gastric acid pH in rats with induced reflux esophagitis (p < 0.05). The treatment with ECR significantly reduced the production of cytokines TNF-α, IL-1β and IL-6 levels compared to the model group (p < 0.05). The expression of TNF-α and COX2 in the intact esophageal mucosa was low while those of the RE control group were significantly higher due to an inflammatory reaction in the esophagus. Compare to the model group, treatment with α-tocopherol or ECR significantly inhibited the expression levels of COX2 and TNF-α in a dose-dependent manner. These results suggest that anti-inflammatory and protective effects of ECR could attenuate the severity of reflux esophagitis and prevent esophageal mucosal damage.
这项研究旨在探讨仙茅根茎（仙茅根茎）对幽门和前胃结扎术诱导的大鼠急性反流性食管炎（RE）的影响。测定促炎细胞因子以及肿瘤坏死因子（TNF）-α、白细胞介素（IL）-1β和 IL-6，并通过 RT-PCR 分析 TNF-α 和 COX2 的表达。与正常完整组相比，反流性食管炎大鼠的食管组织损伤增加。然而，仙茅根茎提取物（ECR）600mg/kg 和 ECR 300mg/kg 的食管损伤百分比明显低于 RE 对照组。诱导反流性食管炎大鼠给予α-生育酚（30mg/kg）和 ECR（600mg/kg、300mg/kg 和 150mg/kg）可显著影响胃内酸 pH 值（p<0.05）。与模型组相比，ECR 治疗可显著降低 TNF-α、IL-1β 和 IL-6 水平的细胞因子产生（p<0.05）。由于食管炎症，完整食管黏膜中 TNF-α 和 COX2 的表达水平较低，而 RE 对照组的表达水平明显较高。与模型组相比，α-生育酚或 ECR 的治疗可显著抑制 COX2 和 TNF-α 的表达水平，呈剂量依赖性。这些结果表明，ECR 的抗炎和保护作用可减轻反流性食管炎的严重程度并防止食管黏膜损伤。
