Department of Chemistry, Division of Food Chemistry and Toxicology, Molecular Nutrition, University of Kaiserslautern, Kaiserslautern, Germany.
Biotechnol J. 2013 Mar;8(3):363-70. doi: 10.1002/biot.201200303. Epub 2013 Jan 17.
Polyphenols contained in food have various positive effects on human health. The absorption and metabolism of polyphenols in the intestinal tract needs to be studied to estimate these effects. The Ussing chamber technique was used to investigate the transport behavior of apple polyphenols through pig small intestinal mucosa, which served as a model for human gastrointestinal mucosa. The identities and concentrations of polyphenols and their metabolites in the half-chambers (luminal and basolateral) within an incubation period of 4 h were determined by HPLC-MS/MS and HPLC-DAD (DAD = diode-array detection). Flux values were also measured. It was found that 5-caffeoylquinic acid and caffeic acid were absorbed and translocated to the basolateral side (1.9 and 3.7%, respectively), but other compounds, including glycosides of phloretin and quercetin, were observed without translocation. A Ussing chamber utilizing pig small intestinal mucosa is a suitable model for assessing the effect of apple polyphenols on mucosal integrity and nutrition absorption across porcine mucosa.
食物中的多酚对人类健康有多种积极影响。需要研究多酚在肠道中的吸收和代谢，以评估这些影响。本研究采用 Ussing 室技术，以猪小肠黏膜为模型，研究苹果多酚在肠道中的转运行为。在 4 小时孵育期内，使用 HPLC-MS/MS 和 HPLC-DAD（DAD = 二极管阵列检测）测定半室（腔侧和基底外侧）中多酚及其代谢物的种类和浓度，并测量通量值。结果表明，5-咖啡酰奎宁酸和咖啡酸被吸收并转运到底侧（分别为 1.9%和 3.7%），但其他化合物，包括根皮苷和槲皮素的糖苷，没有观察到转运。利用猪小肠黏膜的 Ussing 室是评估苹果多酚对黏膜完整性和营养吸收的影响的合适模型。
