Pelin-Enlund K, Husgafvel-Pursiainen K, Tammilehto L, Klockars M, Jantunen K, Gerwin B I, Harris C C, Tuomi T, Vanhala E, Mattson K
Institute of Occupational Health, Department of Industrial Hygiene, Helsinki, Finland.
Carcinogenesis. 1990 Apr;11(4):673-81. doi: 10.1093/carcin/11.4.673.
Seven mesothelioma cell lines were established from clinical specimens from five patients with asbestos-related malignant pleural mesothelioma. The cells in culture show either epithelial or mixed epithelial/fibrosarcomatous growth with an average doubling time of 30 h. Giant multinucleated cells are common in all the cell lines, as well as long thin microvilli on the cell surfaces. All cell lines were cytokeratin positive and they stained negatively for monocyte-macrophage markers. All seven cell lines and one long-term tissue culture from a sixth mesothelioma patient were characterized cytogenetically. Karyotype analyses revealed complex structural and numerical abnormalities, primarily involving chromosome 1, 4, 5, 6, 7, 8, 9, 11, 12, 13 and 22. An excess of chromosome material of the short arm of chromosome 5 was seen consistently in six cell lines and in the long-term culture. In cell lines from four patients, changes in chromosome 13, mainly monosomy 13, were observed. The marker chromosomes observed in the early passages were conserved and few additional changes appeared in later passages. Six of the cell lines tested for tumorigenicity in athymic nude mice were weakly positive.
从5例石棉相关恶性胸膜间皮瘤患者的临床标本中建立了7个间皮瘤细胞系。培养的细胞呈现上皮样生长或上皮/纤维肉瘤混合样生长，平均倍增时间为30小时。所有细胞系中均常见巨大多核细胞，细胞表面还有细长的微绒毛。所有细胞系细胞角蛋白呈阳性，单核细胞-巨噬细胞标志物染色呈阴性。对所有7个细胞系以及第6例间皮瘤患者的一个长期组织培养物进行了细胞遗传学特征分析。核型分析显示存在复杂的结构和数目异常，主要涉及1、4、5、6、7、8、9、11、12、13和22号染色体。在6个细胞系和长期培养物中均一致观察到5号染色体短臂的染色体物质过量。在4例患者的细胞系中观察到13号染色体的变化，主要是13号染色体单体。在早期传代中观察到的标记染色体得以保留，在后期传代中几乎没有出现其他变化。对6个细胞系进行无胸腺裸鼠致瘤性检测，结果呈弱阳性。
