de Cupis A, Pirani P, Favoni R E
Department of Preclinical Oncology, Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
Monaldi Arch Chest Dis. 1998 Apr;53(2):188-92.
Malignant mesothelioma is an aggressive and rare tumor of the mesothelium which arises in serosal cavities and is strongly related to asbestos exposure. The incidence of this tumor is still increasing because of the widespread use of asbestos over the past years and the long latency of this malignancy. The paucity of well-characterized in vitro human models and the consequent shortage of experimental pharmacological studies on mesothelioma reduce the success of its clinical management. It is well known that established human cell lines represent a rapid and advantageous system for in vitro studies of several diseases, such as mesothelioma. Thus, the aim of our study was to establish and characterize a panel of selected human malignant mesothelioma cell lines in order to highlight the biology of this tumor and to test the effectiveness of new antiproliferative compounds. During the last year, we collected 10 pleural effusion samples from patients with diagnosed and pharmacologically untreated mesothelioma. Among these, we were able to isolate three continuously growing cell lines, identified as IST-Mes1, IST-Mes2 and IST-Mes3. Another previously established malignant mesothelioma cell line (MPP89) has also been included in this study. The cells in culture appeared morphologically heterogeneous: three of them (IST-Mes1, IST-Mes2, and MPP89) were spindle-shaped, and upon reaching confluence, they assumed the characteristic cobblestone-like pattern, whereas IST-Mes3 showed mixed sizes and shapes. Cell growth studies revealed that all cell lines reach exponential growth phase within 4-7 days after plating with a doubling time ranging from 37-87 hours. Finally, insulin-like growth factor-I did not stimulate cellular proliferation of both the IST-Mes1 and IST-Mes2 cell lines.
恶性间皮瘤是一种侵袭性的罕见间皮肿瘤，起源于浆膜腔，与石棉暴露密切相关。由于过去几年石棉的广泛使用以及这种恶性肿瘤的长潜伏期，该肿瘤的发病率仍在上升。缺乏特征明确的体外人类模型以及随之而来的间皮瘤实验药理学研究的短缺，降低了其临床管理的成功率。众所周知，已建立的人类细胞系是研究多种疾病（如间皮瘤）体外研究的快速且有利的系统。因此，我们研究的目的是建立并表征一组选定的人类恶性间皮瘤细胞系，以突出这种肿瘤的生物学特性并测试新的抗增殖化合物的有效性。在过去一年中，我们从诊断为间皮瘤且未进行药物治疗的患者中收集了10份胸腔积液样本。在这些样本中，我们能够分离出三个持续生长的细胞系，分别鉴定为IST-Mes1、IST-Mes2和IST-Mes3。另一个先前建立的恶性间皮瘤细胞系（MPP89）也被纳入了本研究。培养的细胞在形态上表现出异质性：其中三个（IST-Mes1、IST-Mes2和MPP89）呈纺锤形，达到汇合后呈现出特征性的鹅卵石样形态，而IST-Mes3则表现出大小和形状的混合。细胞生长研究表明，所有细胞系在接种后4 - 7天内达到指数生长期，倍增时间为37 - 87小时。最后，胰岛素样生长因子-I并未刺激IST-Mes1和IST-Mes2细胞系的细胞增殖。
