新型干燥提取物 BNO 1011 可刺激人呼吸道上皮细胞培养中的氯离子转运和纤毛摆动频率。

The novel dry extract BNO 1011 stimulates chloride transport and ciliary beat frequency in human respiratory epithelial cultures.

机构信息

Children's Hospital of Philadelphia and Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA.

出版信息

Am J Rhinol Allergy. 2012 Nov-Dec;26(6):439-43. doi: 10.2500/ajra.2012.26.3821.

DOI:10.2500/ajra.2012.26.3821

PMID:23232192

Abstract

BACKGROUND

Herbal remedies predate written history and continue to be used more frequently than conventional pharmaceutical medications. The novel dry extract BNO 1011 is based on a combination of five herbs that is used to treat acute and chronic rhinosinusitis. We evaluated the pharmacologic effects of the novel dry extract BNO 1011 on human respiratory epithelial cultures specifically addressing electrolyte transport and cilia beat frequency (CBF).

METHODS

Well-differentiated human bronchial epithelial cultures grown at an air-liquid interface were treated on the apical or basolateral surface with varying concentrations of dry extract BNO 1011. Changes in transepithelial sodium and chloride transport were determined in Ussing chambers under voltage-clamped conditions. Changes in CBF were determined using the Sissons-Ammons Video Analysis system (Ammons Engineering, Mt. Morris, MI).

RESULTS

When applied to the apical surface, dry extract BNO 1011 activated forskolin-stimulated chloride secretion and ciliary beat in a dose-dependent fashion. Basolateral application of dry extract BNO 1011 did not alter the measured physiological properties.

CONCLUSION

Apical application of dry extract BNO 1011 stimulates both chloride secretion and CBF and therefore may augment mucociliary clearance.

摘要

背景

草药疗法的历史可以追溯到有文字记载之前，并且比传统的药物治疗更频繁地被使用。新型干燥提取物 BNO 1011 基于五种草药的组合，用于治疗急性和慢性鼻-鼻窦炎。我们评估了新型干燥提取物 BNO 1011 对人呼吸道上皮细胞培养物的药理作用，特别是针对电解质转运和纤毛摆动频率（CBF）。

方法

在气-液界面培养的分化良好的人支气管上皮细胞分别用不同浓度的干燥提取物 BNO 1011 处理细胞的顶侧或基底外侧表面。在电压钳条件下使用 Ussing 室测定跨上皮钠离子和氯离子转运的变化。使用 Sissons-Ammons Video Analysis 系统（Ammons Engineering，Mt. Morris，MI）测定 CBF 的变化。