Department of Frontier Bioscience and Research Center for Micro-Nano Technology, Hosei University, Koganei, Tokyo, 184-8584, Japan.
Microbiologyopen. 2012 Dec;1(4):381-94. doi: 10.1002/mbo3.42. Epub 2012 Oct 8.
The FixJ/LuxR family transcription factor CsgD is a master regulator of biofilm formation in Escherichia coli. Previously, we identified more than 10 transcription factors that participate in regulation of the csgD promoter. After genomic SELEX screening of regulation targets, an uncharacterized TetR-type transcription factor YbjK was found to be involved in regulation of the csgD promoter. In addition, a number of stress-response genes were found to be under the direct control of YbjK. Taken together, we propose to rename it to RcdA (regulator of csgD). One unique feature of RcdA is its mode of DNA binding. Gel shift, DNase-I footprinting, and atomic force microscopic (AFM) analyses indicated that RcdA is a DNA-binding protein with a high level of cooperativity, with which it covers the entire surface of probe DNA through protein-protein interaction and moreover it induces the formation of aggregates of DNA-RcdA complexes.
FixJ/LuxR 家族转录因子 CsgD 是大肠杆菌生物膜形成的主要调节剂。之前，我们鉴定了 10 多个参与 csgD 启动子调控的转录因子。在对调控靶点进行基因组 SELEX 筛选后，发现一个未被表征的 TetR 型转录因子 YbjK 参与了 csgD 启动子的调控。此外，还发现许多应激反应基因受 YbjK 的直接控制。综上所述，我们建议将其重新命名为 RcdA（csgD 调控因子）。RcdA 的一个独特特征是其 DNA 结合模式。凝胶迁移、DNase-I 足迹和原子力显微镜 (AFM) 分析表明，RcdA 是一种具有高协同性的 DNA 结合蛋白，通过蛋白质-蛋白质相互作用覆盖探针 DNA 的整个表面，并且它还诱导 DNA-RcdA 复合物聚集的形成。
