Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia.
ACS Nano. 2013 Jan 22;7(1):522-30. doi: 10.1021/nn3046117. Epub 2012 Dec 12.
Particle shape is emerging as a key design parameter for tailoring the interactions between particles and cells. Herein, we report the preparation of rod-shaped layer-by-layer (LbL)-assembled polymer hydrogel capsules with tunable aspect ratios (ARs). By templating spherical and rodlike silica particles, disulfide-stabilized poly(methacrylic acid) hydrogel capsules (PMA HCs) with different ARs (from 1 to 4) are generated. The influence of capsule AR on cellular internalization and intracellular fate was quantitatively investigated by flow cytometry, imaging flow cytometry, and fluorescence deconvolution microscopy. These experiments reveal that the cellular internalization kinetics of PMA HCs are dependent on the AR, with spherical capsules being internalized more rapidly and to a greater extent compared with rod-shaped capsules. In contrast, the capsules with different ARs are colocalized with the lysosomal marker LAMP1, suggesting that the lysosomal compartmentalization is independent of shape for these soft polymer capsules.
颗粒形状正在成为设计用于调整颗粒与细胞之间相互作用的关键参数。在此，我们报告了具有可调纵横比（AR）的棒状层层（LbL）组装聚合物水凝胶胶囊的制备。通过模板化球形和棒状二氧化硅颗粒，生成了具有不同纵横比（从 1 到 4）的二硫键稳定的聚（甲基丙烯酸）水凝胶胶囊（PMA HC）。通过流式细胞术、成像流式细胞术和荧光反卷积显微镜定量研究了胶囊 AR 对细胞内化和细胞内命运的影响。这些实验表明，PMA HC 的细胞内化动力学取决于 AR，与棒状胶囊相比，球形胶囊的内化速度更快，程度更大。相比之下，不同 AR 的胶囊与溶酶体标记物 LAMP1 共定位，这表明对于这些软聚合物胶囊，溶酶体区室化与形状无关。
