大鼠主动脉的丙烯酰胺处理以及对去氧肾上腺素和钾的反应

Acrylamide-treatment and responses to phenylephrine and potassium in rat aorta.

作者信息

Nurullahoğlu-Atalık E, Okudan N, Belviranlı M, Esen H, Yener Y, Oznurlu Y

机构信息

Department of Pharmacology, University of Konya, Konya, Turkey.

出版信息

Acta Physiol Hung. 2012 Dec;99(4):420-9. doi: 10.1556/APhysiol.99.2012.4.6.

DOI:10.1556/APhysiol.99.2012.4.6

PMID:23238544

Abstract

Acrylamide (ACR) is a chemical used in many industries around the world and was found to form naturally in foods cooked at high temperatures. The aim of the present study is to evaluate the influence of ACR treatment on vascular responses to phenylephrine (PHE; 10-9-3×10-4 M) and potassium chloride (KCl; 5-100 mM). We also examined the role of gender in these responses. The animals in both genders were divided into three groups as follows. (1) Control animals, (2) ACR-I; ACR-treated (2 mg/kg-d for 90 days), (3) ACR-II; ACR-treated (5 mg/kg-d for 90 days). Male rat aortas were more sensitive to PHE and KCl than female aortas. ACR-treatment increased the sensitivity to PHE and KCl, in both genders. Compared to the control group, ACR treatment significantly reduced the luminal area of both male and female rat aortas. Furthermore, the responses to PHE and KCl were similar in both 2 mg/kg-d ACR-treated rat aortas with 5 mg/kg-d ACR-treated rat aortas, in both genders. The results of this study suggest that ACR treatment affects vascular contractility and morphology in both gender of rat aorta.

摘要

丙烯酰胺（ACR）是一种在全球众多行业中使用的化学物质，并且被发现会在高温烹饪的食物中自然形成。本研究的目的是评估ACR处理对血管对去氧肾上腺素（PHE；10⁻⁹ - 3×10⁻⁴ M）和氯化钾（KCl；5 - 100 mM）反应的影响。我们还研究了性别在这些反应中的作用。将两种性别的动物分为以下三组。（1）对照动物，（2）ACR - I组；经ACR处理（2 mg/kg - d，持续90天），（3）ACR - II组；经ACR处理（5 mg/kg - d，持续90天）。雄性大鼠主动脉对PHE和KCl的反应比雌性主动脉更敏感。ACR处理增加了两种性别的大鼠主动脉对PHE和KCl的敏感性。与对照组相比，ACR处理显著减小了雄性和雌性大鼠主动脉的管腔面积。此外，在两种性别中，2 mg/kg - d ACR处理的大鼠主动脉和5 mg/kg - d ACR处理的大鼠主动脉对PHE和KCl的反应相似。本研究结果表明，ACR处理会影响大鼠主动脉两种性别的血管收缩性和形态。