College of Chemistry, Sichuan University, Chengdu 610064, PR China.
Small. 2013 Jun 10;9(11):1929-38, 1928. doi: 10.1002/smll.201201437. Epub 2012 Dec 13.
Upconverting nanoparticles (UCNPs) have attracted considerable attention as potential photosensitizer carriers for photodynamic therapy (PDT) in deep tissues. In this work, a new and efficient NIR photosensitizing nanoplatform for PDT based on red-emitting UCNPs is designed. The red emission band matches well with the efficient absorption bands of the widely used commercially available photosensitizers (Ps), benefiting the fluorescence resonance energy transfer (FRET) from UCNPs to the attached photosensitizers and thus efficiently activating them to generate cytotoxic singlet oxygen. Three commonly used photosensitizers, including chlorine e6 (Ce6), zinc phthalocyanine (ZnPc) and methylene blue (MB), are loaded onto the alpha-cyclodextrin-modified UCNPs to form Ps@UCNPs complexes that efficiently produce singlet oxygen to kill cancer cells under 980 nm near-infrared excitation. Moreover, two different kinds of drugs are co-loaded onto these nanoparticles: chemotherapy drug doxorubicin and PDT agent Ce6. The combinational therapy based on doxorubicin (DOX)-induced chemotherapy and Ce6-triggered PDT exhibits higher therapeutic efficacy relative to the individual means for cancer therapy in vitro.
上转换纳米粒子（UCNPs）作为用于深部组织光动力疗法（PDT）的潜在光敏剂载体，引起了相当大的关注。在这项工作中，设计了一种基于红色发射 UCNPs 的新型高效近红外光敏纳米平台用于 PDT。红色发射带与广泛使用的市售光敏剂（Ps）的有效吸收带很好地匹配，有利于从 UCNPs 到附着的光敏剂的荧光共振能量转移（FRET），从而有效地激活它们以产生细胞毒性单线态氧。三种常用的光敏剂，包括氯 e6（Ce6）、锌酞菁（ZnPc）和亚甲蓝（MB），负载到α-环糊精修饰的 UCNPs 上，形成 Ps@UCNPs 复合物，在 980nm 近红外激发下有效地产生单线态氧来杀死癌细胞。此外，两种不同的药物被共负载到这些纳米颗粒上：化疗药物阿霉素和 PDT 剂 Ce6。基于阿霉素（DOX）诱导的化疗和 Ce6 触发的 PDT 的联合治疗在体外癌症治疗方面表现出比单独治疗更高的疗效。
