Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO, 80303, USA.
Department of Electrical, Computer and Energy Engineering, University of Colorado, Boulder, CO, 80303, USA.
Small. 2017 Jun;13(24). doi: 10.1002/smll.201700504. Epub 2017 May 8.
DNA-mediated assembly of core-satellite structures composed of Zr(IV)-based porphyrinic metal-organic framework (MOF) and NaYF ,Yb,Er upconverting nanoparticles (UCNPs) for photodynamic therapy (PDT) is reported. MOF NPs generate singlet oxygen ( O ) upon photoirradiation with visible light without the need for additional small molecule, diffusional photosensitizers such as porphyrins. Using DNA as a templating agent, well-defined MOF-UCNP clusters are produced where UCNPs are spatially organized around a centrally located MOF NP. Under NIR irradiation, visible light emitted from the UCNPs is absorbed by the core MOF NP to produce O at significantly greater amounts than what can be produced from simply mixing UCNPs and MOF NPs. The MOF-UCNP core-satellite superstructures also induce strong cell cytotoxicity against cancer cells, which are further enhanced by attaching epidermal growth factor receptor targeting affibodies to the PDT clusters, highlighting their promise as theranostic photodynamic agents.
报道了一种 DNA 介导的核-卫星结构的组装，该结构由基于 Zr(IV)的卟啉金属-有机骨架（MOF）和 NaYF4:Yb,Er 上转换纳米粒子（UCNPs）组成，用于光动力疗法（PDT）。MOF NPs 在可见光照射下进行光辐照时会产生单线态氧（1O2），而不需要额外的小分子扩散性光敏剂，如卟啉。使用 DNA 作为模板剂，可以制备出具有明确结构的 MOF-UCNP 簇，其中 UCNPs 围绕位于中心的 MOF NP 进行空间排列。在近红外光照射下，UCNPs 发出的可见光被核心 MOF NP 吸收，从而产生比简单混合 UCNPs 和 MOF NPs 所能产生的更多的 1O2。MOF-UCNP 核-卫星超结构还会对癌细胞产生强烈的细胞毒性，而将表皮生长因子受体靶向 affibodies 连接到 PDT 簇上则进一步增强了这种毒性，这突出了它们作为治疗性光动力剂的应用前景。
