Dipartimento di Chimica, Università degli Studi di Milano, via Golgi 19, 20133 Milano, Italy.
Macromol Biosci. 2013 Mar;13(3):332-47. doi: 10.1002/mabi.201200354. Epub 2012 Dec 13.
This paper reports on the synthesis and physico-chemical, mechanical, and biological characterization of two sets of poly(amidoamine) (PAA) hydrogels with potential as scaffolds for in vivo peripheral nerve regeneration. They are obtained by polyaddition of piperazine with N,N'-methylenebis(acrylamide) or 1,4-bis(acryloyl)piperazine with 1,2-diaminoethane as cross-linking agent and exhibit a combination of relevant properties, such as mechanical strength, biocompatibility, biodegradability, ability to induce adhesion and proliferation of Schwann cells (SCs) preserving their viability. Moreover, the most promising hydrogels, that is those deriving from 1,4-bis(acryloyl)piperazine, allow the in vitro growth of the sensitive neurons of the dorsal root ganglia, thus getting around a critical point in the design of conduits for nerve regeneration.
本文报道了两组具有体内周围神经再生支架潜力的聚（酰胺-胺）（PAA）水凝胶的合成及物理化学、机械和生物学特性。它们是通过哌嗪与 N，N'-亚甲基双（丙烯酰胺）或 1,4-双（丙烯酰基）哌嗪与 1,2-二氨基乙烷作为交联剂的加聚反应得到的，具有相关性能的组合，如机械强度、生物相容性、可生物降解性、诱导雪旺细胞（SCs）黏附和增殖的能力，并保持其活力。此外，最有前途的水凝胶，即那些源自 1,4-双（丙烯酰基）哌嗪的水凝胶，允许背根神经节的敏感神经元在体外生长，从而解决了神经再生导管设计中的一个关键点。
